NDLI: Biogenesis of Iron-Sulfur Clusters in Photosystem I

Content Provider	PubMed Central
Author	Jin, Zhao Heinnickel, Mark Krebs, Carsten Shen, Gaozhong Golbeck, John H. Bryant, Donald A.
Abstract	The NfuA protein has been postulated to act as a scaffolding protein in the biogenesis of photosystem (PS) I and other iron-sulfur (Fe/S) proteins in cyanobacteria and chloroplasts. To determine the properties of NfuA, recombinant NfuA from Synechococcus sp. PCC 7002 was overproduced and purified. In vitro reconstituted NfuA contained oxygen- and EDTA-labile Fe/S cluster(s), which had EPR properties consistent with [4Fe-4S] clusters. After reconstitution with 57Fe2+, Mössbauer studies of NfuA showed a broad quadrupole doublet that confirmed the presence of [4Fe-4S]2+ clusters. Native gel electrophoresis under anoxic conditions and chemical cross-linking showed that holo-NfuA forms dimers and tetramers harboring Fe/S cluster(s). Combined with iron and sulfide analyses, the results indicated that one [4Fe-4S] cluster was bound per NfuA dimer. Fe/S cluster transfer from holo-NfuA to apo-PsaC of PS I was studied by reconstitution of PS I complexes using P700-FX core complexes, PsaD, apo-PsaC, and holo-NfuA. Electron transfer measurements by time-resolved optical spectroscopy showed that holo-NfuA rapidly and efficiently transferred [4Fe-4S] clusters to PsaC in a reaction that required contact between the two proteins. The NfuA-reconstituted PS I complexes had typical charge recombination kinetics from [FA/FB]- to P700+ and light-induced low-temperature EPR spectra. These results establish that cyanobacterial NfuA can act as a scaffolding protein for the insertion of [4Fe-4S] clusters into PsaC of PS I in vitro.
Related Links	http://dx.doi.org/10.1074/jbc.m803395200
Ending Page	28435
Page Count	10
Starting Page	28426
File Format	PDF
ISSN	00219258
e-ISSN	1083351X
Journal	The Journal of Biological Chemistry
Issue Number	42
Volume Number	283
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2008-10-17
Access Restriction	Open
Rights Holder	American Society for Biochemistry and Molecular Biology
Subject Keyword	Cell Biology Biochemistry Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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Biogenesis of Iron-Sulfur Clusters in Photosystem I HOLO-NfuA FROM THE CYANOBACTERIUM SYNECHOCOCCUS SP. PCC 7002 RAPIDLY AND EFFICIENTLY TRANSFERS [4Fe-4S] CLUSTERS TO APO-PsaC IN VITRO

Biogenesis of Iron-Sulfur Clusters in Photosystem I: HOLO-NfuA FROM THE CYANOBACTERIUM SYNECHOCOCCUS SP. PCC 7002 RAPIDLY AND EFFICIENTLY TRANSFERS [4Fe-4S] CLUSTERS TO APO-PsaC IN VITRO

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