NDLI: Analyzing the Interaction of RseA and RseB, the Two Negative Regulators of the σE Envelope Stress Response, Using a Combined Bioinformatic and Experimental Strategy* S⃞

Content Provider	PubMed Central
Author	Ahuja, Nidhi Korkin, Dmitry Chaba, Rachna Cezairliyan, Brent O. Sauer, Robert T. Kim, Kyeong Kyu Gross, Carol A.
Abstract	The Escherichia coli envelope stress response is controlled by the alternative sigma factor, σE, and is induced when unfolded outer membrane proteins accumulate in the periplasm. The response is initiated by sequential cleavage of the membrane-spanning antisigma factor, RseA. RseB is an important negative regulator of envelope stress response that exerts its negative effects onσE activity through its binding to RseA. In this study, we analyze the interaction between RseA and RseB. We found that tight binding of RseB to RseA required intact RseB. Using programs that performed global and local sequence alignment of RseB and RseA, we found regions of high similarity and performed alanine substitution mutagenesis to test the hypothesis that these regions were functionally important. This protocol is based on the hypothesis that functionally dependent regions of two proteins co-evolve and therefore are likely to be sequentially conserved. This procedure allowed us to identify both an N-terminal and C-terminal region in RseB important for binding to RseA. We extensively analyzed the C-terminal region, which aligns with a region of RseA coincident with the major RseB binding determinant in RseA. Both allele-specific suppression analysis and cysteine-mediated disulfide bond formation indicated that this C-terminal region of similarity of RseA and RseB identifies a contact site between the two proteins. We suggest a similar protocol can be successfully applied to pairs of non-homologous but functionally linked proteins to find specific regions of the protein sequences that are important for establishing functional linkage.
Related Links	http://dx.doi.org/10.1074/jbc.m806012200
Ending Page	5413
Page Count	11
Starting Page	5403
File Format	PDF
ISSN	00219258
e-ISSN	1083351X
Journal	The Journal of Biological Chemistry
Issue Number	8
Volume Number	284
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2009-02-20
Access Restriction	Open
Rights Holder	American Society for Biochemistry and Molecular Biology
Subject Keyword	Cell Biology Biochemistry Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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Analyzing the Interaction of RseA and RseB, the Two Negative Regulators of the σE Envelope Stress Response, Using a Combined Bioinformatic and Experimental Strategy

Analyzing the interaction of RseA and RseB, the two negative regulators of the sigmaE envelope stress response, using a combined bioinformatic and experimental strategy.

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Analyzing the Interaction of RseA and RseB, the Two Negative Regulators of the σE Envelope Stress Response, Using a Combined Bioinformatic and Experimental Strategy* S⃞

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