NDLI: Analytical Performance of Immobilized Pronase for Glycopeptide Footprinting and Implications for Surpassing Reductionist Glycoproteomics

Please wait, while we are loading the content...

Proteomics-determined differences in the Concanavalin-A-fractionated proteome of hippocampus and inferior parietal lobule in subjects with Alzheimer’s disease and mild cognitive impairment: Implications for progression of AD

Pancreatic Cancer Serum Detection Using A Lectin/Glyco-Antibody Array Method

Exploiting Differential Dissociation Chemistries of O-Linked Glycopeptide Ions for the Localization of Mucin-Type Protein Glycosylation

Analytical Performance of Immobilized Pronase for Glycopeptide Footprinting and Implications for Surpassing Reductionist Glycoproteomics

Identification and development of fucosylated glycoproteins as biomarkers of primary hepatocellular carcinoma

Glycoproteomics for Prostate Cancer Detection: Changes in Serum PSA Glycosylation Patterns

Glycomic Characterization of Prostate Specific Antigen and Prostatic Acid Phosphatase in Prostate Cancer and Benign Disease Seminal Plasma Fluids

Elucidation of O-glycosylation structures of the ß-amyloid precursor protein by liquid chromatography - mass spectrometry using electron transfer dissociation and collision induced dissociation

Mapping Glycans onto Specific N-Linked Glycosylation Sites of Pyrus Communis PGIP Redefines the Interface for EPG:PGIP Interactions

A Perspective on the Maillard Reaction and the Analysis of Protein Glycation by Mass Spectrometry: Probing the Pathogenesis of Chronic Disease

Mass spectrometry based targeted protein quantification: methods and applications

Phosphorylation Site Mapping of Endogenous Proteins: A Combined MS and Bioinformatics Approach

Identification and Analysis of Occludin Phosphosites: A Combined Mass Spectrometry and Bioinformatics Approach

Comparison of MS2-only, MSA, and MS2/MS3 Methodologies for Phosphopeptide Identification

Identification and Verification of Lysine Propionylation and Butyrylation in Yeast Core Histones Using PTMap Software

High-Throughput Liquid-Liquid Fractionation of Multiple Protein Post-Translational Modifications*

EVALUATION OF ARCHIVAL TIME ON SHOTGUN PROTEOMICS OF FORMALIN-FIXED AND PARAFFIN-EMBEDDED TISSUES

Proteomic Analysis and Identification of the Structural and Regulatory Proteins of the Rhodobacter capsulatus Gene Transfer Agent

Prion Disease Diagnosis by Proteomic Profiling

Absolute Quantification of C-Reactive Protein in Human Plasma Derived from Patients with Epithelial Ovarian Cancer Utilizing Protein Cleavage Isotope Dilution Mass Spectrometry

Year: 2009, Volume: 8, Issue: 1

Year: 2008, Volume: 7

Year: 2007, Volume: 6

Year: 2006, Volume: 5

Year: 2005, Volume: 4

Year: 2004, Volume: 3

Year: 2002, Volume: 1

Analytical Performance of Immobilized Pronase for Glycopeptide Footprinting and Implications for Surpassing Reductionist Glycoproteomics

Content Provider	PubMed Central
Author	Dodds, Eric D. Seipert, Richard R. Clowers, Brian H. German, J. Bruce Lebrilla, Carlito B.
Abstract	A fully developed understanding of protein glycosylation requires characterization of the modifying oligosaccharides, elucidation of their covalent attachment sites, and determination of the glycan heterogeneity at specific sites. Considering the complexity inherent to protein glycosylation, establishing these features for even a single protein can present an imposing challenge. In order to meet the demands of glycoproteomics, the capability to screen far more complex systems of glycosylated proteins must be developed. Although the proteome wide examination of carbohydrate modification has become an area of keen interest, the intricacy of protein glycosylation has frustrated the progress of large scale, systems oriented research on site-specific protein-glycan relationships. Indeed, the analytical obstacles in this area have been more instrumental in shaping the current glycoproteomic paradigm than have the diverse functional roles and ubiquitous nature of glycans. This report describes the ongoing development and analytically salient features of bead immobilized pronase for glycosylation site footprinting. The present work bears on the ultimate goal of providing analytical tools capable of addressing the diversity of protein glycosylation in a more comprehensive and efficient manner. In particular, this approach has been assessed with respect to reproducibility, sensitivity, and tolerance to sample complexity. The efficiency of pronase immobilization, attainable pronase loading density, and the corresponding effects on glycoprotein digestion rate were also evaluated. In addition to being highly reproducible, the immobilized enzymes retained a high degree of proteolytic activity after repeat usage for up to 6 weeks. This method also afforded a low level of chemical background and provided favorable levels of sensitivity with respect to traditional glycoproteomic strategies. Thus, the application of immobilized pronase shows potential to contribute to the advancement of more comprehensive glycoproteomic research methods that are capable of providing site-specific glycosylation and microheterogeneity information across many proteins.
Related Links	http://dx.doi.org/10.1021/pr800708h
Ending Page	512
Page Count	11
Starting Page	502
File Format	PDF
ISSN	15353893
e-ISSN	15353907
Journal	Journal of proteome research
Issue Number	2
Volume Number	8
Language	English
Publisher Date	2009-02-06
Access Restriction	Open
Subject Keyword	Biochemistry Chemistry(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Chemistry Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

It is all about the solvent: on the importance of the mobile phase for ZIC-HILIC glycopeptide enrichment

It is all about the solvent: on the importance of the mobile phase for ZIC-HILIC glycopeptide enrichment

The Art of Destruction: Optimizing Collision Energies in Quadrupole-Time of Flight (Q-TOF) Instruments for Glycopeptide-Based Glycoproteomics

Deciphering the bacterial glycocode: recent advances in bacterial glycoproteomics

Development of Immobilized Enzyme Reactors for the characterization of the glycosylation heterogeneity of a protein

Glycoproteomics in Neurodegenerative Diseases

Community Evaluation of Glycoproteomics Informatics Solutions Reveals High-Performance Search Strategies of Serum N- and O-Glycopeptide Data

Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers

Optimizing Performance of Glycopeptide Capture for Plasma Proteomics

Analytical Performance of Immobilized Pronase for Glycopeptide Footprinting and Implications for Surpassing Reductionist Glycoproteomics

Similar Documents

It is all about the solvent: on the importance of the mobile phase for ZIC-HILIC glycopeptide enrichment

It is all about the solvent: on the importance of the mobile phase for ZIC-HILIC glycopeptide enrichment

The Art of Destruction: Optimizing Collision Energies in Quadrupole-Time of Flight (Q-TOF) Instruments for Glycopeptide-Based Glycoproteomics

Deciphering the bacterial glycocode: recent advances in bacterial glycoproteomics

Development of Immobilized Enzyme Reactors for the characterization of the glycosylation heterogeneity of a protein

Glycoproteomics in Neurodegenerative Diseases

Community Evaluation of Glycoproteomics Informatics Solutions Reveals High-Performance Search Strategies of Serum N- and O-Glycopeptide Data

Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers

Optimizing Performance of Glycopeptide Capture for Plasma Proteomics

Analytical Performance of Immobilized Pronase for Glycopeptide Footprinting and Implications for Surpassing Reductionist Glycoproteomics