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| Content Provider | PubMed Central |
|---|---|
| Author | O'rand, Michael G. Widgren, Esther E. Beyler, Stan Richardson, Richard T. |
| Copyright Year | 2009 |
| Abstract | Epididymal protease inhibitor (eppin [official symbol, SPINLW1]) is of interest as a male contraceptive target because of its specificity and location on the human sperm surface. We have examined the effect of anti-eppin antibodies from infertile male monkeys and the effect of recombinant human semenogelin on human sperm motility. Anti-eppin antibodies significantly decreased the progressive motility of human spermatozoa as measured by decreased total distance traveled, decreased straight-line distance, and decreased velocity. Anti-eppin treatment of spermatozoa significantly increased the amount of cAMP present in nonprogressive spermatozoa; however, approximately 25% of antibody-treated spermatozoa could be rescued by the addition of cAMP-acetoxymethyl ester, indicating that anti-eppin-treated spermatozoa have a compromised ability to utilize cAMP. Addition of recombinant human semenogelin has a concentration-dependent inhibitory effect on progressive motility (increased tortuosity and decreased velocity). We tested the hypothesis that anti-eppin antibodies bound to eppin would subsequently block semenogelin binding to eppin. Anti-eppin antibodies from infertile monkeys inhibited eppin from binding to semenogelin. Addition of affinity-purified antibodies made to the dominant C-terminal epitope of eppin had an inhibitory effect on progressive motility (increased tortuosity, decreased velocity, and straight distance). Our results suggest that the eppin-semenogelin binding site is critical for the removal of semenogelin in vivo during semen liquefaction and for the initiation of progressive motility. We conclude that the eppin-semenogelin binding site on the surface of human spermatozoa is an ideal target for a nonsteroidal male contraceptive. |
| Related Links | http://dx.doi.org/10.1095/biolreprod.108.072942 |
| Ending Page | 285 |
| Page Count | 7 |
| Starting Page | 279 |
| File Format | |
| ISSN | 00063363 |
| e-ISSN | 15297268 |
| Journal | Biology of Reproduction |
| Issue Number | 2 |
| Volume Number | 80 |
| Language | English |
| Publisher | Society for the Study of Reproduction, Inc. |
| Publisher Date | 2009-02-01 |
| Access Restriction | Open |
| Rights Holder | Society for the Study of Reproduction, Inc. |
| Subject Keyword | Cell Biology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Reproductive Medicine |
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