NDLI: Spatial Calibration of Structured Illumination Fluorescence Microscopy using Capillary Tissue Phantoms

Please wait, while we are loading the content...

Spatial Calibration of Structured Illumination Fluorescence Microscopy using Capillary Tissue Phantoms

Content Provider	PubMed Central
Author	Lee, Grace S. Miele, Lino F. Turhan, Aslihan Lin, Miao Dusan, Hanidziar Konerding, Moritz A. Mentzer, Steven J.
Abstract	Quantitative assessment of microvascular structure is relevant to the investigations of ischemic injury, reparative angiogenesis and tumor revascularization. In light microscopy applications, thick tissue specimens are necessary to characterize microvascular networks; however, thick tissue leads to image distortions due to out-of-focus light. Structured illumination confocal microscopy is an optical sectioning technique that improves contrast and resolution by using a grid pattern to identify the plane-of-focus within the specimen. Because structured illumination can be applied to wide-field (nonscanning) microscopes, the microcirculation can be studied by sequential intravital and confocal microscopy. To assess the application of structured illumination confocal microscopy to microvessel imaging, we studied cell-sized microspheres and fused silica microcapillary tissue phantoms. As expected, structured illumination produced highly accurate images in the lateral (X-Y) plane, but demonstrated a loss of resolution in the Z-Y plane. Because the magnitude of Z-axis distortion was variable in complex tissues, the silica microcapillaries were used as spatial calibration standards. Morphometric parameters, such as shape factor, were used to empirically optimize Z-axis software compression. We conclude that the silica microcapillaries provide a useful tissue phantom for in vitro studies as well as spatial calibration standard for in vivo morphometry of the microcirculation.
Related Links	http://dx.doi.org/10.1002/jemt.20647
Ending Page	92
Page Count	8
Starting Page	85
File Format	PDF
ISSN	1059910X
e-ISSN	10970029
Journal	Microscopy research and technique
Issue Number	2
Volume Number	72
Language	English
Publisher Date	2009-02-01
Access Restriction	Open
Subject Keyword	Anatomy Instrumentation Medical Laboratory Technology Histology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Anatomy Histology Instrumentation Medical Laboratory Technology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Spatial calibration of structured illumination fluorescence microscopy using capillary tissue phantoms.

Combining fluorescence and bioluminescence microscopy

Inclined Selective Plane Illumination Microscopy (iSPIM) adaptor for conventional microscopes

High-Resolution Solid Modeling of Biological Samples Imaged with 3D Fluorescence Microscopy

A deep tissue fluorescence imaging system with enhanced SHG detection capabilities

Biosensor FRET detection by the phasor approach to fluorescence lifetime imaging microscopy (FLIM)

Widefield fluorescence microscopy with extended resolution

Development of an optimized protocol for studying the interaction of filamentous bacteriophage with mammalian cells by fluorescence microscopy

A Combined Atomic Force/Fluorescence Microscopy Technique to Select Aptamers in a Single Cycle From a Small Pool of Random Oligonucleotides

Spatial Calibration of Structured Illumination Fluorescence Microscopy using Capillary Tissue Phantoms

Similar Documents

Spatial calibration of structured illumination fluorescence microscopy using capillary tissue phantoms.

Combining fluorescence and bioluminescence microscopy

Inclined Selective Plane Illumination Microscopy (iSPIM) adaptor for conventional microscopes

High-Resolution Solid Modeling of Biological Samples Imaged with 3D Fluorescence Microscopy

A deep tissue fluorescence imaging system with enhanced SHG detection capabilities

Biosensor FRET detection by the phasor approach to fluorescence lifetime imaging microscopy (FLIM)

Widefield fluorescence microscopy with extended resolution

Development of an optimized protocol for studying the interaction of filamentous bacteriophage with mammalian cells by fluorescence microscopy

A Combined Atomic Force/Fluorescence Microscopy Technique to Select Aptamers in a Single Cycle From a Small Pool of Random Oligonucleotides

Spatial Calibration of Structured Illumination Fluorescence Microscopy using Capillary Tissue Phantoms