NDLI: Dictyostelium Dock180-related RacGEFs Regulate the Actin Cytoskeleton during Cell Motility

Content Provider	PubMed Central
Author	Para, Alessia Krischke, Miriam Sylvain, Merlot Shen, Zhouxin Oberholzer, Michael Lee, Susan Briggs, Steven Firtel, Richard A.
Editor	Parent, Carole
Copyright Year	2009
Abstract	Cell motility of amoeboid cells is mediated by localized F-actin polymerization that drives the extension of membrane protrusions to promote forward movements. We show that deletion of either of two members of the Dictyostelium Dock180 family of RacGEFs, DockA and DockD, causes decreased speed of chemotaxing cells. The phenotype is enhanced in the double mutant and expression of DockA or DockD complements the reduced speed of randomly moving DockD null cells' phenotype, suggesting that DockA and DockD are likely to act redundantly and to have similar functions in regulating cell movement. In this regard, we find that overexpressing DockD causes increased cell speed by enhancing F-actin polymerization at the sites of pseudopod extension. DockD localizes to the cell cortex upon chemoattractant stimulation and at the leading edge of migrating cells and this localization is dependent on PI3K activity, suggesting that DockD might be part of the pathway that links PtdIns(3,4,5)P3 production to F-actin polymerization. Using a proteomic approach, we found that DdELMO1 is associated with DockD and that Rac1A and RacC are possible in vivo DockD substrates. In conclusion, our work provides a further understanding of how cell motility is controlled and provides evidence that the molecular mechanism underlying Dock180-related protein function is evolutionarily conserved.
Related Links	http://dx.doi.org/10.1091/mbc.E08-09-0899
Starting Page	699
File Format	PDF
ISSN	19394586
e-ISSN	19394586
Journal	Molecular Biology of the Cell
Issue Number	2
Volume Number	20
Language	English
Publisher	The American Society for Cell Biology
Publisher Date	2009-01-15
Access Restriction	Open
Rights Holder	The American Society for Cell Biology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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