NDLI: G0 Function of BCL2 and BCL-xL Requires BAX, BAK, and p27 Phosphorylation by Mirk, Revealing a Novel Role of BAX and BAK in Quiescence Regulation*

Content Provider	PubMed Central
Author	Yelena, Janumyan Cui, Qinghua Yan, Ling Sansam, Courtney G. Valentin, Mayda Yang, Elizabeth
Abstract	BCL2 and BCL-xL facilitate G0 quiescence by decreasing RNA content and cell size and up-regulating p27 protein, but the precise mechanism is not understood. We investigated the relationship between cell cycle regulation and the anti-apoptosis function of BCL2 and BCL-xL. Neither caspase inhibition nor abrogation of mitochondria-dependent apoptosis by BAX and BAK deletion fully recapitulated the G0 effects of BCL2 or BCL-xL, suggesting that mechanisms in addition to anti-apoptosis are involved in the cell cycle arrest function of BCL2 or BCL-xL. We found that BCL2 and BCL-xL expression in bax -/- bak -/- cells did not confer cell cycle effects, consistent with the G0 function of BCL2 and BCL-xL being mediated through BAX or BAK. Stabilization of p27 in G0 in BCL2 or BCL-xL cells was due to phosphorylation of p27 at Ser10 by the kinase Mirk. In bax -/- bak -/- cells, total p27 and p27 phosphorylated at Ser10 were elevated. Re-expression of BAX in bax -/- bak -/- cells and silencing of BAX and BAK in wild type cells confirmed that endogenous BAX and BAK modulated p27. These data revealed a novel role for BAX and BAK in the regulation of G0 quiescence.
Related Links	http://dx.doi.org/10.1074/jbc.m806294200
Ending Page	34120
Page Count	13
Starting Page	34108
File Format	PDF
ISSN	00219258
e-ISSN	1083351X
Journal	The Journal of Biological Chemistry
Issue Number	49
Volume Number	283
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2008-12-05
Access Restriction	Open
Rights Holder	American Society for Biochemistry and Molecular Biology
Subject Keyword	Cell Biology Biochemistry Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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G0 Function of BCL2 and BCL-xL Requires BAX, BAK, and p27 Phosphorylation by Mirk, Revealing a Novel Role of BAX and BAK in Quiescence Regulation

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G0 Function of BCL2 and BCL-xL Requires BAX, BAK, and p27 Phosphorylation by Mirk, Revealing a Novel Role of BAX and BAK in Quiescence Regulation*

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