NDLI: An autologous oral DNA vaccine protects against murine melanoma

Content Provider	PubMed Central
Author	Xiang, Rong Lode, Holger N. Chao, Ta-hsiang Ruehlmann, J. Michael Dolman, Carrie S. Rodriguez, Fernando Whitton, J. Lindsay Overwijk, Willem W. Restifo, Nicholas P. Reisfeld, Ralph A.
Copyright Year	2000
Abstract	We demonstrated that peripheral T cell tolerance toward murine melanoma self-antigens gp100 and TRP-2 can be broken by an autologous oral DNA vaccine containing the murine ubiquitin gene fused to minigenes encoding peptide epitopes gp10025–33 and TRP-2181–188. These epitopes contain dominant anchor residues for MHC class I antigen alleles H-2Db and H-2Kb, respectively. The DNA vaccine was delivered by oral gavage by using an attenuated strain of Salmonella typhimurium as carrier. Tumor-protective immunity was mediated by MHC class I antigen-restricted CD8+ T cells that secreted TH1 cytokine IFN-γ and induced tumor rejection and growth suppression after a lethal challenge with B16G3.26 murine melanoma cells. Importantly, the protective immunity induced by this autologous DNA vaccine against murine melanoma cells was at least equal to that achieved through xenoimmunization with the human gp10025–33 peptide, which differs in its three NH2-terminal amino acid residues from its murine counterpart and was previously reported to be clearly superior to an autologous vaccine in inducing protective immunity. The presence of ubiquitin upstream of the minigene proved to be essential for achieving this tumor-protective immunity, suggesting that effective antigen processing and presentation may make it possible to break peripheral T cell tolerance to a self-antigen. This vaccine design might prove useful for future rational designs of other recombinant DNA vaccines targeting tissue differentiation antigens expressed by tumors.
Related Links	http://dx.doi.org/10.1073/pnas.090097697
Ending Page	5497
Page Count	6
Starting Page	5492
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	10
Volume Number	97
Language	English
Publisher	The National Academy of Sciences
Publisher Date	2000-05-09
Access Restriction	Open
Rights Holder	The National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

An autologous oral DNA vaccine protects against murine melanoma.

A consensus–hemagglutinin-based DNA vaccine that protects mice against divergent H5N1 influenza viruses

A nonreplicating subunit vaccine protects mice against lethal Ebola virus challenge

A second-generation vaccine protects against the psychoactive effects of cocaine

DNA vaccine coding for the full-length infectious Kunjin virus RNA protects mice against the New York strain of West Nile virus

Immunization by vaccine-coated microneedle arrays protects against lethal influenza virus challenge

Cytolytic T-cell clones against an autologous human melanoma: specificity study and definition of three antigens by immunoselection.

A Novel Murine Cytomegalovirus Vaccine Vector Protects against Mycobacterium tuberculosis

Highly attenuated smallpox vaccine protects mice with and without immune deficiencies against pathogenic vaccinia virus challenge

An autologous oral DNA vaccine protects against murine melanoma

Similar Documents

An autologous oral DNA vaccine protects against murine melanoma.

A consensus–hemagglutinin-based DNA vaccine that protects mice against divergent H5N1 influenza viruses

A nonreplicating subunit vaccine protects mice against lethal Ebola virus challenge

A second-generation vaccine protects against the psychoactive effects of cocaine

DNA vaccine coding for the full-length infectious Kunjin virus RNA protects mice against the New York strain of West Nile virus

Immunization by vaccine-coated microneedle arrays protects against lethal influenza virus challenge

Cytolytic T-cell clones against an autologous human melanoma: specificity study and definition of three antigens by immunoselection.

A Novel Murine Cytomegalovirus Vaccine Vector Protects against Mycobacterium tuberculosis

Highly attenuated smallpox vaccine protects mice with and without immune deficiencies against pathogenic vaccinia virus challenge

An autologous oral DNA vaccine protects against murine melanoma