NDLI: Mosaic DNA Imports with Interspersions of Recipient Sequence after Natural Transformation of Helicobacter pylori

Content Provider	PubMed Central
Author	Kulick, Stefan Moccia, Claudia Didelot, Xavier Daniel, Falush Kraft, Christian Sebastian, Suerbaum
Editor	Ahmed, Niyaz
Copyright Year	2008
Abstract	Helicobacter pylori colonizes the gastric mucosa of half of the human population, causing gastritis, ulcers, and cancer. H. pylori is naturally competent for transformation by exogenous DNA, and recombination during mixed infections of one stomach with multiple H. pylori strains generates extensive allelic diversity. We developed an in vitro transformation protocol to study genomic imports after natural transformation of H. pylori. The mean length of imported fragments was dependent on the combination of donor and recipient strain and varied between 1294 bp and 3853 bp. In about 10% of recombinant clones, the imported fragments of donor DNA were interrupted by short interspersed sequences of the recipient (ISR) with a mean length of 82 bp. 18 candidate genes were inactivated in order to identify genes involved in the control of import length and generation of ISR. Inactivation of the antimutator glycosylase MutY increased the length of imports, but did not have a significant effect on ISR frequency. Overexpression of mutY strongly increased the frequency of ISR, indicating that MutY, while not indispensable for ISR formation, is part of at least one ISR-generating pathway. The formation of ISR in H. pylori increases allelic diversity, and contributes to the uniquely low linkage disequilibrium characteristic of this pathogen.
Related Links	http://dx.doi.org/10.1371/journal.pone.0003797
Starting Page	3797
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	11
Volume Number	3
Language	English
Publisher	Public Library of Science
Publisher Date	2008-11-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Multiple Pathways of Plasmid DNA Transfer in Helicobacter pylori

Risk Assessment of Gastric Cancer Caused by Helicobacter pylori Using CagA Sequence Markers

The Characterization of Helicobacter pylori DNA Associated with Ancient Human Remains Recovered from a Canadian Glacier

Functional Analysis of an Acid Adaptive DNA Adenine Methyltransferase from Helicobacter pylori 26695

Infection with Helicobacter pylori Is Associated with Protection against Tuberculosis

Are Helicobacter Pylori and Other Helicobacter Species Infection Associated with Human Biliary Lithiasis? A Meta-Analysis

Helicobacter pylori Seropositivity and Risk of Lung Cancer

Biochemical Characterization of Hypothetical Proteins from Helicobacter pylori

Elevated Interleukin-32 Expression Is Associated with Helicobacter pylori-Related Gastritis

Mosaic DNA Imports with Interspersions of Recipient Sequence after Natural Transformation of Helicobacter pylori

Similar Documents

Multiple Pathways of Plasmid DNA Transfer in Helicobacter pylori

Risk Assessment of Gastric Cancer Caused by Helicobacter pylori Using CagA Sequence Markers

The Characterization of Helicobacter pylori DNA Associated with Ancient Human Remains Recovered from a Canadian Glacier

Functional Analysis of an Acid Adaptive DNA Adenine Methyltransferase from Helicobacter pylori 26695

Infection with Helicobacter pylori Is Associated with Protection against Tuberculosis

Are Helicobacter Pylori and Other Helicobacter Species Infection Associated with Human Biliary Lithiasis? A Meta-Analysis

Helicobacter pylori Seropositivity and Risk of Lung Cancer

Biochemical Characterization of Hypothetical Proteins from Helicobacter pylori

Elevated Interleukin-32 Expression Is Associated with Helicobacter pylori-Related Gastritis

Mosaic DNA Imports with Interspersions of Recipient Sequence after Natural Transformation of Helicobacter pylori