NDLI: Mechanistic Investigations of the Pseudouridine Synthase RluA using RNA containing 5-Fluorouridine†

Content Provider	PubMed Central
Author	Hamilton, Christopher S. Greco, Todd M. Vizthum, Caroline A. Ginter, Joy M. Johnston, Murray V. Mueller, Eugene G.
Abstract	The pseuoduridine synthases (Ψ synthases) isomerize uridine (U) to pseudouridine (Ψ) in RNA, and they fall into five families that share very limited sequence similarity but have the same overall fold and active site architecture, including an essential Asp. The mechanism by which the Ψ synthases operate remains unknown, and mechanistic work has largely made use of RNA containing 5-fluorouridine (f5U) in place of U. The Ψ synthase TruA forms a covalent adduct with such RNA, and heat disruption of the adduct generates a hydrated product of f5U, which was reasonably concluded to result from the hydrolysis of an ester linkage between the essential Asp and f5U. In contrast, the Ψ synthase TruB, which is a member of a different family, does not form an adduct with f5U in RNA but catalyzes the rearrangement and hydration of the f5U, which labeling studies with [18O]water showed does not result from ester hydrolysis. To extend the line of mechanistic investigation to another family of Ψ synthases and an enzyme that makes an adduct with f5U in RNA, the behavior of RluA towards RNA containing f5U was examined. Stem-loop RNAs are shown to be good substrates for RluA. Heat denaturation of the adduct between RluA and RNA containing f5U produces a hydrated nucleoside product, and labeling studies show that hydration does not occur by ester hydrolysis. These results are interpreted in light of a consistent mechanistic scheme for the handling of f5U by Ψ synthases.
Related Links	http://dx.doi.org/10.1021/bi061293x
Ending Page	12038
Page Count	10
Starting Page	12029
File Format	PDF
ISSN	00062960
e-ISSN	15204995
Journal	Biochemistry
Issue Number	39
Volume Number	45
Language	English
Publisher Date	2006-10-01
Access Restriction	Open
Subject Keyword	Biochemistry Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biochemistry

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The handling of the mechanistic probe 5-fluorouridine by the pseudouridine synthase TruA and its consistency with the handling of the same probe by the pseudouridine synthases TruB and RluA†

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