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| Content Provider | PubMed Central |
|---|---|
| Author | Song, Liyan Ren, Shengfang Yu, Rongmin Yan, Chunyan Li, Tingfei Zhao, Yu |
| Copyright Year | 2008 |
| Abstract | Two purified proteins G-6 and G-4-2 were obtained from Arca subcrenata Lischke using the homogenization, salting-out with ammonium sulfate, ion-exchange chromatography and gel filtration chromatography techniques. The purity of G-6 and G-4-2 was over 96%, as measured by RP-HPLC. G-6 and G-4-2 were measured by SDS-PAGE and IEF-PAGE to have molecular weights of 8.2 kDa and 16.0 kDa, and isoelectric points of 6.6 and 6.1, respectively. The amino acid constituents of G-6 and G-4-2 were also determined. The existence of saccharides in G-6 was demonstrated by the phenol-sulfuric acid method. G-6 and G-4-2 inhibited the proliferation of human tumor cells in vitro. By MTT assay, the IC50 values of G-4-2 were 22.9 μg/mL, 46.1 μg/mL and 57.7 μg/mL against Hela, HL-60 and KB cell lines, respectively, and the IC50 value of G-6 against HL-60 cell line was measured to be 123.2 μg/mL. |
| Related Links | http://dx.doi.org/10.3390/md6030418 |
| Ending Page | 430 |
| Page Count | 13 |
| Starting Page | 418 |
| File Format | |
| ISSN | 16603397 |
| e-ISSN | 16603397 |
| Journal | Marine Drugs |
| Issue Number | 3 |
| Volume Number | 6 |
| Language | English |
| Publisher | Molecular Diversity Preservation International |
| Publisher Date | 2008-07-01 |
| Access Restriction | Open |
| Rights Holder | Molecular Diversity Preservation International |
| Subject Keyword | Drug Discovery Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Pharmaceutical Science |
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