NDLI: Metal ion specificities for folding and cleavage activity in the Schistosoma hammerhead ribozyme

Content Provider	PubMed Central
Author	Boots, Jennifer L. Canny, Marella D. Azimi, Ehsan Pardi, Arthur
Copyright Year	2008
Abstract	The effects of various metal ions on cleavage activity and global folding have been studied in the extended Schistosoma hammerhead ribozyme. Fluorescence resonance energy transfer was used to probe global folding as a function of various monovalent and divalent metal ions in this ribozyme. The divalent metals ions Ca2+, Mg2+, Mn2+, and Sr2+ have a relatively small variation (less than sixfold) in their ability to globally fold the hammerhead ribozyme, which contrasts with the very large difference (>10,000-fold) in apparent rate constants for cleavage for these divalent metal ions in single-turnover kinetic experiments. There is still a very large range (>4600-fold) in the apparent rate constants for cleavage for these divalent metal ions measured in high salt (2 M NaCl) conditions where the ribozyme is globally folded. These results demonstrate that the identity of the divalent metal ion has little effect on global folding of the Schistosoma hammerhead ribozyme, whereas it has a very large effect on the cleavage kinetics. Mechanisms by which the identity of the divalent metal ion can have such a large effect on cleavage activity in the Schistosoma hammerhead ribozyme are discussed.
Related Links	http://dx.doi.org/10.1261/rna.1010808
Ending Page	2222
Page Count	11
Starting Page	2212
File Format	PDF
ISSN	13558382
e-ISSN	14699001
Journal	RNA
Issue Number	10
Volume Number	14
Language	English
Publisher	Cold Spring Harbor Laboratory Press
Publisher Date	2008-08-01
Access Restriction	Open
Rights Holder	Cold Spring Harbor Laboratory Press
Subject Keyword	Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology

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Metal Ion-Mediated Folding and Catalysis of the Hammerhead Ribozyme

A two-metal ion mechanism operates in the hammerhead ribozyme-mediated cleavage of an RNA substrate

The ion-induced folding of the hammerhead ribozyme: core sequence changes that perturb folding into the active conformation.

Explanation by the double-metal-ion mechanism of catalysis for the differential metal ion effects on the cleavage rates of 5′-oxy and 5′-thio substrates by a hammerhead ribozyme

Efficient trans-cleavage by the Schistosoma mansoni SMα1 hammerhead ribozyme in the extreme thermophile Thermus thermophilus

Metal ion binding and the folding of the hairpin ribozyme.

Correlation between hammerhead ribozyme-mediated eggshell protein gene cleavage and reproduction inhibition of Schistosoma japonicum

Specificity of hammerhead ribozyme cleavage.

Dissection of the ion-induced folding of the hammerhead ribozyme using 19F NMR

Metal ion specificities for folding and cleavage activity in the Schistosoma hammerhead ribozyme

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Metal Ion-Mediated Folding and Catalysis of the Hammerhead Ribozyme

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