NDLI: Interaction between the Helicobacter pylori accessory proteins HypA and UreE is needed for urease maturation

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Lipid composition and transcriptional response of Mycobacterium tuberculosis grown under iron-limitation in continuous culture: identification of a novel wax ester

Interaction between the Helicobacter pylori accessory proteins HypA and UreE is needed for urease maturation

Year: 2007, Volume: 153, Issue: Pt 4

Year: 2007, Volume: 153, Issue: Pt 2

Year: 2007, Volume: 153, Issue: 0 3

Year: 2006, Volume: 152

Year: 2005, Volume: 151

Year: 2004, Volume: 150

Year: 2000, Volume: 146

Interaction between the Helicobacter pylori accessory proteins HypA and UreE is needed for urease maturation

Content Provider	PubMed Central
Author	Benoit, Stéphane L. Mehta, Nalini Weinberg, Michael V. Maier, Cheryl Maier, Robert J.
Abstract	Several accessory proteins are required for the maturation of two nickel-containing enzymes in the gastric pathogen Helicobacter pylori. These two enzymes are hydrogenase and urease. Among the accessory/maturation proteins, the nickel-binding HypA protein has been previously shown to be required for the full activity of both the hydrogenase and the urease enzymes, while another nickel-binding protein, UreE, is known to be solely involved in the urease maturation process. In this study, UreE was shown to be required under all nickel levels for full activation of the apourease. By use of cross-linking studies, an interaction between purified HypA and UreE proteins was identified, leading to the formation of a 34 kDa heterodimer complex. The cross-linked adduct was detected by immunoblotting with either anti-HypA or anti-UreE antiserum. By using a two-plasmid system in Escherichia coli, the highest urease activity was achieved under low nickel conditions only when the UreE protein was expressed along with the wild-type HypA protein, but not with its nickel-binding-deficient variant HypA H2A. Addition of only 1 µM NiCl2 into minimal medium abolished the need for HypA to activate the urease. Although various attempts to show direct nickel transfer from HypA to UreE failed, these results suggest that interactions between the nickel-binding accessory proteins HypA and UreE are required to allow nickel transfer from HypA eventually to the apourease in H. pylori.
Related Links	http://dx.doi.org/10.1099/mic.0.2006/003228-0
Ending Page	1482
Page Count	9
Starting Page	1474
File Format	PDF
ISSN	13500872
e-ISSN	14652080
Journal	Microbiology (Reading, England)
Issue Number	Pt 5
Volume Number	153
Language	English
Publisher Date	2007-05-01
Access Restriction	Open
Subject Keyword	Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Microbiology

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Helicobacter pylori hydrogenase accessory protein HypA and urease accessory protein UreG compete with each other for UreE recognition

Characterization of Helicobacter pylori Nickel Metabolism Accessory Proteins Needed for Maturation of both Urease and Hydrogenase

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Interaction between the Helicobacter pylori accessory proteins HypA and UreE is needed for urease maturation

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