NDLI: Identification of parasite DNA in common bile duct stones by PCR and DNA sequencing

Content Provider	PubMed Central
Author	Jang, Ji Sun Kim, Kyung Ho Yu, Jae-ran Lee, Soo-ung
Copyright Year	2007
Abstract	We attempted to identify parasite DNA in the biliary stones of humans via PCR and DNA sequencing. Genomic DNA was isolated from each of 15 common bile duct (CBD) stones and 5 gallbladder (GB) stones. The patients who had the CBD stones suffered from cholangitis, and the patients with GB stones showed acute cholecystitis, respectively. The 28S and 18S rDNA genes were amplified successfully from 3 and/or 1 common bile duct stone samples, and then cloned and sequenced. The 28S and 18S rDNA sequences were highly conserved among isolates. Identity of the obtained 28S D1 rDNA with that of Clonorchis sinensis was higher than 97.6%, and identity of the 18S rDNA with that of other Ascarididae was 97.9%. Almost no intra-specific variations were detected in the 28S and 18S rDNA with the exception of a few nucleotide variations, i.e., substitution and deletion. These findings suggest that C. sinensis and Ascaris lumbricoides may be related with the biliary stone formation and development.
Related Links	http://dx.doi.org/10.3347/kjp.2007.45.4.301
Starting Page	301
File Format	PDF
ISSN	00234001
e-ISSN	17380006
Journal	The Korean Journal of Parasitology
Issue Number	4
Volume Number	45
Language	English
Publisher	The Korean Society for Parasitology
Publisher Date	2007-01-01
Access Restriction	Open
Rights Holder	The Korean Society for Parasitology
Subject Keyword	Parasitology Infectious Diseases Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology

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