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| Content Provider | PubMed Central |
|---|---|
| Author | Bastia, Deepak Zzaman, Shamsu Krings, Gregor Saxena, Mukesh Peng, Xiaohua Greenberg, Marc M. |
| Abstract | The replication terminator protein Tus of Escherichia coli promotes polar fork arrest at sequence-specific replication termini (Ter) by antagonizing DNA unwinding by the replicative helicase DnaB. Here, we report that Tus is also a polar antitranslocase. We have used this activity as a tool to uncouple helicase arrest at a Tus–Ter complex from DNA unwinding and have shown that helicase arrest occurred without the generation of a DNA fork or a bubble of unpaired bases at the Tus–Ter complex. A mutant form of Tus, which reduces DnaB–Tus interaction but not the binding affinity of Tus for Ter DNA, was also defective in arresting a sliding DnaB. A model of polar fork arrest that proposes melting of the Tus–Ter complex and flipping of a conserved C residue of Ter at the blocking but not the nonblocking face has been reported. The model suggests that enhanced stability of Tus–Ter interaction caused by DNA melting and capture of a flipped base by Tus generates polarity strictly by enhanced protein–DNA interaction. In contrast, the observations presented here show that polarity of helicase and fork arrest in vitro is generated by a mechanism that not only involves interaction between the terminator protein and the arrested enzyme but also of Tus with Ter DNA, without any melting and base flipping in the termination complex. |
| Related Links | http://dx.doi.org/10.1073/pnas.0805898105 |
| Ending Page | 12836 |
| Page Count | 6 |
| Starting Page | 12831 |
| File Format | |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 35 |
| Volume Number | 105 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2008-09-02 |
| Access Restriction | Open |
| Rights Holder | National Academy of Sciences |
| Subject Keyword | General Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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