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| Content Provider | PubMed Central |
|---|---|
| Author | Flamand, Louis Gravel, Annie David, Boutolleau Roberto, Alvarez-lafuente Jacobson, Steve Malnati, Mauro S. Kohn, Debra Tang, Yi-wei Yoshikawa, Tetsushi Ablashi, Dharam |
| Abstract | Human herpesvirus 6 (HHV-6) is a ubiquitous virus with which infections have been associated with pathologies ranging from delayed bone marrow engraftment to a variety of neurological diseases. The lack of a standardized assay that can be used to detect and estimate HHV-6 DNA contents in various clinical specimens can lead and has led to discordant results among investigators and on the potential association of HHV-6 to diseases. To identify the most reliable and sensitive assays, an identical set of 11 coded serum samples spiked with various quantities of the HHV-6A variant (range, 4 to 400,000 genome copies/ml) was sent to eight independent laboratories around the world. Each laboratory was asked to estimate the HHV-6 DNA content by use of its own protocols and assays. Among the various assays, three TaqMan-based real-time PCR assays yielded quantities that were closest to the quantity of HHV-6 that had been spiked. To provide better homogeneity between the results from the different laboratories working on HHV-6, we propose that investigators interested in quantifying HHV-6 in clinical samples adopt one of these assays. |
| Related Links | http://dx.doi.org/10.1128/jcm.00370-08 |
| Ending Page | 2706 |
| Page Count | 7 |
| Starting Page | 2700 |
| File Format | |
| ISSN | 00951137 |
| e-ISSN | 1098660X |
| Journal | Journal of Clinical Microbiology |
| Issue Number | 8 |
| Volume Number | 46 |
| Language | English |
| Publisher | American Society for Microbiology (ASM) |
| Publisher Date | 2008-08-01 |
| Access Restriction | Open |
| Rights Holder | American Society for Microbiology (ASM) |
| Subject Keyword | Microbiology (medical) Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology (medical) |
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