NDLI: Processing the nonstructural polyproteins of sindbis virus: nonstructural proteinase is in the C-terminal half of nsP2 and functions both in cis and in trans.

Please wait, while we are loading the content...

Year: 2016, Volume: 90

Year: 2015, Volume: 90

Year: 2015, Volume: 89

Year: 2014, Volume: 89

Year: 2014, Volume: 88

Year: 2013, Volume: 87

Year: 2012, Volume: 86

Year: 2011, Volume: 85

Year: 2010, Volume: 84

Year: 2009, Volume: 83

Year: 2008, Volume: 82

Year: 2007, Volume: 81

Year: 2006, Volume: 80

Year: 2005, Volume: 79

Year: 2004, Volume: 78

Year: 2003, Volume: 77

Year: 2002, Volume: 76

Year: 2001, Volume: 75

Year: 2000, Volume: 74

Year: 1999, Volume: 73

Year: 1998, Volume: 72

Year: 1997, Volume: 71

Year: 1996, Volume: 70

Year: 1995, Volume: 69

Year: 1994, Volume: 68

Year: 1993, Volume: 67

Year: 1992, Volume: 66

Year: 1991, Volume: 65

Year: 1990, Volume: 64

Year: 1989, Volume: 63

Year: 1989, Volume: 63, Issue: 12

Year: 1989, Volume: 63, Issue: 11

A novel function of the herpes simplex virus type 1 Fc receptor: participation in bipolar bridging of antiviral immunoglobulin G.

A baculovirus gene with a novel transcription pattern encodes a polypeptide with a zinc finger and a leucine zipper.

Persistent infection of rabbits with bovine leukemia virus associated with development of immune dysfunction.

A single point mutation of Ala-25 to Asp in the 14,000-Mr envelope protein of vaccinia virus induces a size change that leads to the small plaque size phenotype of the virus.

Transforming activity of a 16-amino-acid segment of the bovine papillomavirus E5 protein linked to random sequences of hydrophobic amino acids.

Respective roles of pyrimidine dimer and pyrimidine (6-4) pyrimidone photoproducts in UV mutagenesis of simian virus 40 DNA in mammalian cells.

Passive immunity modulates genetic reassortment between rotaviruses in mixedly infected mice.

The cellular proteins which can associate specifically with polyomavirus middle T antigen in human 293 cells include the major human 70-kilodalton heat shock proteins.

UV-induced transcription from the human immunodeficiency virus type 1 (HIV-1) long terminal repeat and UV-induced secretion of an extracellular factor that induces HIV-1 transcription in nonirradiated cells.

Effects of deletions in the N-terminal basic arm of brome mosaic virus coat protein on RNA packaging and systemic infection.

Receptor activity of rotavirus nonstructural glycoprotein NS28.

Multiple isoelectric forms of poliovirus RNA-dependent RNA polymerase: evidence for phosphorylation.

Beta and gamma interferons act synergistically to produce an antiviral state in cells resistant to both interferons individually.

Herpes simplex virus type 1 ICP0 plays a critical role in the de novo synthesis of infectious virus following transfection of viral DNA.

The regions important for the activator and repressor functions of herpes simplex virus type 1 alpha protein ICP27 map to the C-terminal half of the molecule.

Avian-to-human transmission of the PB1 gene of influenza A viruses in the 1957 and 1968 pandemics.

Identification and characterization of an Epstein-Barr virus early antigen that is encoded by the NotI repeats.

Multiple transcriptional regulatory domains in the human immunodeficiency virus type 1 long terminal repeat are involved in basal and E1A/E1B-induced promoter activity.

Integrated proviral human immunodeficiency virus type 1 is present in CD4+ peripheral blood lymphocytes in healthy seropositive individuals.

Transcription of a poxvirus early gene is regulated both by a short promoter element and by a transcriptional termination signal controlling transcriptional interference.

A recombinant hepatitis B core antigen polypeptide with the protamine-like domain deleted self-assembles into capsid particles but fails to bind nucleic acids.

Processing the nonstructural polyproteins of sindbis virus: nonstructural proteinase is in the C-terminal half of nsP2 and functions both in cis and in trans.

Expression of infectious woodchuck hepatitis virus in murine and avian fibroblasts.

Mutational analysis of the cleavage sequence of the human immunodeficiency virus type 1 envelope glycoprotein precursor gp160.

Proteolytic digestion of reovirus in the intestinal lumens of neonatal mice.

Evidence for interference, coinfections, and intertypic virus enhancement of infection by ovine-caprine lentiviruses.

Inhibition of rRNA synthesis by poliovirus: specific inactivation of transcription factors.

Use of Ar+ plasma etching to localize structural proteins in the capsid of herpes simplex virus type 1.

A comparison of the genome organization of capripoxvirus with that of the orthopoxviruses.

Significance of premature stop codons in env of simian immunodeficiency virus.

A host cell protein binds to a highly conserved sequence element (pac-2) within the cytomegalovirus a sequence.

Degradation of cellular proteins during poliovirus infection: studies by two-dimensional gel electrophoresis.

Analysis of T4 bacteriophage deletion mutants that lack td and frd genes.

Antigenic properties and cellular localization of herpes simplex virus glycoprotein H synthesized in a mammalian cell expression system.

Selective spread of herpes simplex virus in the central nervous system after ocular inoculation.

Locations of amino acid substitutions in bacteriophage T4 tsL56 DNA polymerase predict an N-terminal exonuclease domain.

Structure and cell surface maturation of the attachment glycoprotein of human respiratory syncytial virus in a cell line deficient in O glycosylation.

Linker insertion mutants of simian virus 40 large T antigen that show trans-dominant interference with wild-type large T antigen map to multiple sites within the T-antigen gene.

A mutation in the short 5'-proximal open reading frame on Rous sarcoma virus RNA alters virus production.

Partial reversion of conditional transformation correlates with a decrease in the sensitivity of rat cells to killing by the parvovirus minute virus of mice but not in their capacity for virus production: effect of a temperature-sensitive v-src oncogene.

Protection of mice from herpes simplex virus-induced retinitis by in vitro-activated immune cells.

A GC-box motif upstream of the B19 parvovirus unique promoter is important for in vitro transcription.

Requirement of the single base insertion at the 3' end of the env-related gene of Friend spleen focus-forming virus for pathogenic activity and its effect on localization of the glycoprotein product (gp55).

The herpes simplex virus virion host shutoff function.

Derivation and characterization of an efficiently myocarditic reovirus variant.

The reovirus M1 gene, encoding a viral core protein, is associated with the myocarditic phenotype of a reovirus variant.

Transfer of a mutant dihydrofolate reductase gene into pre- and postimplantation mouse embryos by a replication-competent retrovirus vector.

Effects of mutations in poliovirus 3Dpol on RNA polymerase activity and on polyprotein cleavage.

Analysis of rev gene function on human immunodeficiency virus type 1 replication in lymphoid cells by using a quantitative polymerase chain reaction method.

Spatial and temporal distribution of bovine herpesvirus 1 transcripts.

Polyomavirus genome and polyomavirus enhancer-driven gene expression during myogenesis.

Heterogeneity of the human papillomavirus group.

Human papillomavirus type 27: detection of a novel human papillomavirus in common warts of a renal transplant recipient.

Human papillomavirus type 28 (HPV-28), an HPV-3-related type associated with skin warts.

Human papillomavirus type 29 (HPV-29), an HPV type cross-hybridizing with HPV-2 and with HPV-3-related types.

Human papillomavirus type 48.

Human papillomavirus type 49, a type isolated from flat warts of renal transplant patients.

Human papillomavirus (HPV) type 50, a type associated with epidermodysplasia verruciformis (EV) and only weakly related to other EV-specific HPVs.

Human papillomavirus type 53.

In vitro mutagenesis of the herpes simplex virus type 1 DNA polymerase gene results in altered drug sensitivity of the enzyme.

The NF-kappa B binding sites in the human immunodeficiency virus type 1 long terminal repeat are not required for virus infectivity.

Analysis of regulatory elements of the equine infectious anemia virus and caprine arthritis-encephalitis virus long terminal repeats.

Characterization of a simian hepatitis A virus (HAV): antigenic and genetic comparison with human HAV.

Relative rates of RNA synthesis across the genome of Epstein-Barr virus are highest near oriP and oriLyt.

Differential DNA binding of nuclear proteins to a long terminal repeat region of the MCF13 and Akv murine leukemia viruses.

Nucleotide sequence of a neurovirulent variant of the type 2 oral poliovirus vaccine.

Antibody reactivity to different regions of human T-cell leukemia virus type 1 gp61 in infected people.

A common site for immortalizing proviral integrations in Friend erythroleukemia: molecular cloning and characterization.

Mutational analysis of bovine papillomavirus type 1 E5 peptide domains involved in induction of cellular DNA synthesis.

Duck hepatitis B virus can tolerate insertion, deletion, and partial frameshift mutation in the distal pre-S region.

Antiviral effect of lymphokine-activated killer cells: chemotaxis and homing to sites of virus infection.

Expression of the int-1 and int-2 loci in endogenous mouse mammary tumor virus-induced mammary tumorigenesis in the C3Hf mouse.

Latent infections in spinal ganglia with thymidine kinase-deficient herpes simplex virus.

cis-acting sequences that control the level of viral DNA synthesis in the polyomavirus late region.

Ancestry of a human endogenous retrovirus family.

Effects of alterations in the leader sequence of Rous sarcoma virus RNA on initiation of translation.

Year: 1989, Volume: 63, Issue: 10

Year: 1989, Volume: 63, Issue: 9

Year: 1989, Volume: 63, Issue: 8

Year: 1989, Volume: 63, Issue: 7

Year: 1989, Volume: 63, Issue: 6

Year: 1989, Volume: 63, Issue: 5

Year: 1989, Volume: 63, Issue: 4

Year: 1989, Volume: 63, Issue: 3

Year: 1989, Volume: 63, Issue: 2

Year: 1989, Volume: 63, Issue: 1

Year: 1988, Volume: 62

Year: 1987, Volume: 61

Year: 1986, Volume: 60

Year: 1986, Volume: 59

Year: 1986, Volume: 58

Year: 1986, Volume: 57

Year: 1985, Volume: 56

Year: 1985, Volume: 55

Year: 1985, Volume: 54

Year: 1985, Volume: 53

Year: 1984, Volume: 52

Year: 1984, Volume: 51

Year: 1984, Volume: 50

Year: 1984, Volume: 49

Year: 1983, Volume: 48

Year: 1983, Volume: 47

Year: 1983, Volume: 46

Year: 1983, Volume: 45

Year: 1982, Volume: 44

Year: 1982, Volume: 43

Year: 1982, Volume: 42

Year: 1982, Volume: 41

Year: 1981, Volume: 40

Year: 1981, Volume: 39

Year: 1981, Volume: 38

Year: 1981, Volume: 37

Year: 1980, Volume: 36

Year: 1980, Volume: 35

Year: 1980, Volume: 34

Year: 1980, Volume: 33

Year: 1979, Volume: 32

Year: 1979, Volume: 31

Year: 1979, Volume: 30

Year: 1979, Volume: 29

Year: 1978, Volume: 28

Year: 1978, Volume: 27

Year: 1978, Volume: 26

Year: 1978, Volume: 25

Year: 1977, Volume: 24

Year: 1977, Volume: 23

Year: 1977, Volume: 22

Year: 1977, Volume: 21

Year: 1976, Volume: 20

Year: 1976, Volume: 19

Year: 1976, Volume: 18

Year: 1976, Volume: 17

Year: 1975, Volume: 16

Year: 1975, Volume: 15

Year: 1974, Volume: 14

Year: 1974, Volume: 13

Year: 1973, Volume: 12

Year: 1973, Volume: 11

Year: 1972, Volume: 10

Year: 1972, Volume: 9

Year: 1971, Volume: 8

Year: 1971, Volume: 7

Year: 1970, Volume: 6

Year: 1970, Volume: 5

Year: 1969, Volume: 4

Year: 1969, Volume: 3

Year: 1968, Volume: 2

Year: 1967, Volume: 1

Processing the nonstructural polyproteins of sindbis virus: nonstructural proteinase is in the C-terminal half of nsP2 and functions both in cis and in trans.

Content Provider	PubMed Central
Author	Hardy, W. R. Strauss, J. H.
Abstract	The processing of the Sindbis virus nonstructural polyprotein translated in vitro has been studied. When Sindbis virus genomic RNA was translated in a reticulocyte lysate, polyprotein P123 was cleaved efficiently to produce nsP1, nsP2, and nsP3. Inhibition of this processing by anti-nsP2 antibodies, but not by antibodies specific for nsP1, nsP3, or nsP4, suggested that the viral proteinase was present in nsP2. To localize the proteolytic activity more precisely, deletions were made in a full-length cDNA clone of Sindbis virus, and RNA was transcribed from these constructs with SP6 RNA polymerase and translated in vitro. Although virtually all of the nsP1, nsP3, and nsP4 sequences could be deleted without affecting processing, deletions in the N-terminal half of nsP2 led to aberrant processing, and deletions in the C-terminal half abolished proteolysis. However, inactive polyproteins containing the nsP2 deletions could be processed by exogenously supplied proteins translated from virion RNA, demonstrating that cleavage was virus specific and not due to a protease present in the reticulocyte lysate and that the deleted polyproteins still served as substrates for the enzyme. From these results and from experiments in which processing was studied at increasingly higher dilution, we have concluded the following: (i) the viral nonstructural proteinase is located in the C-terminal half of nsP2; (ii) in the P123 precursor the cleavage between nsP2 and nsP3 occurs efficiently as a bimolecular reaction (in trans) to remove nsP3, while the bond between nsP1 and nsP2 is cleaved inefficiently, but detectably, in trans, but no autoproteolysis of P123 was detected; (iii) once nsP3 has been removed, the bond between nsP1 and nsP2 in the P12 precursor is cleaved efficiently by autoproteolysis (in cis). This mode of processing leads to a slow rate of cleavage, particularly early in infection, suggesting that the polyproteins might play roles in virus RNA replication distinct from those of the cleaved products. A hypothesis is presented that the proteinase is a thiol protease related to papain.
Starting Page	4653
File Format	PDF
ISSN	10985514
e-ISSN	10985514
Journal	Journal of Virology
Issue Number	11
Volume Number	63
Language	English
Publisher Date	1989-11-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology Insect Science

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in