NDLI: Opposite Effects of Mn2+ and Zn2+ on PsaR-Mediated Expression of the Virulence Genes pcpA, prtA, and psaBCA of Streptococcus pneumoniae ▿

Content Provider	PubMed Central
Author	Kloosterman, Tomas G. Witwicki, Robert M. Van, Der Kooi-pol Magdalena M. Bijlsma, Jetta J. E. Kuipers, Oscar P.
Abstract	Homeostasis of Zn2+ and Mn2+ is important for the physiology and virulence of the human pathogen Streptococcus pneumoniae. Here, transcriptome analysis was used to determine the response of S. pneumoniae D39 to a high concentration of Zn2+. Interestingly, virulence genes encoding the choline binding protein PcpA, the extracellular serine protease PrtA, and the Mn2+ uptake system PsaBC(A) were strongly upregulated in the presence of Zn2+. Using random mutagenesis, a previously described Mn2+-responsive transcriptional repressor, PsaR, was found to mediate the observed Zn2+-dependent derepression. In addition, PsaR is also responsible for the Mn2+-dependent repression of these genes. Subsequently, we investigated how these opposite effects are mediated by the same regulator. In vitro binding of purified PsaR to the prtA, pcpA, and psaB promoters was stimulated by Mn2+, whereas Zn2+ destroyed the interaction of PsaR with its target promoters. Mutational analysis of the pcpA promoter demonstrated the presence of a PsaR operator that mediates the transcriptional effects. In conclusion, PsaR is responsible for the counteracting effects of Mn2+ and Zn2+ on the expression of several virulence genes in S. pneumoniae, suggesting that the ratio of these metal ions exerts an important influence on pneumococcal pathogenesis.
Related Links	http://dx.doi.org/10.1128/jb.00307-08
Ending Page	5393
Page Count	12
Starting Page	5382
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	15
Volume Number	190
Language	English
Publisher	American Society for Microbiology (ASM)
Publisher Date	2008-08-01
Access Restriction	Open
Rights Holder	American Society for Microbiology (ASM)
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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