NDLI: Extracellular Charge Adsorption Influences Intracellular Electrochemical Homeostasis in Amphibian Skeletal Muscle

Content Provider	PubMed Central
Author	Mehta, Arpan R. Huang, Christopher L. H. Skepper, Jeremy N. Fraser, James A.
Abstract	The membrane potential measured by intracellular electrodes, E m, is the sum of the transmembrane potential difference (E 1) between inner and outer cell membrane surfaces and a smaller potential difference (E 2) between a volume containing fixed charges on or near the outer membrane surface and the bulk extracellular space. This study investigates the influence of E 2 upon transmembrane ion fluxes, and hence cellular electrochemical homeostasis, using an integrative approach that combines computational and experimental methods. First, analytic equations were developed to calculate the influence of charges constrained within a three-dimensional glycocalyceal matrix enveloping the cell membrane outer surface upon local electrical potentials and ion concentrations. Electron microscopy confirmed predictions of these equations that extracellular charge adsorption influences glycocalyceal volume. Second, the novel analytic glycocalyx formulation was incorporated into the charge-difference cellular model of Fraser and Huang to simulate the influence of extracellular fixed charges upon intracellular ionic homeostasis. Experimental measurements of E m supported the resulting predictions that an increased magnitude of extracellular fixed charge increases net transmembrane ionic leak currents, resulting in either a compensatory increase in Na+/K+-ATPase activity, or, in cells with reduced Na+/K+-ATPase activity, a partial dissipation of transmembrane ionic gradients and depolarization of E m.
Related Links	http://dx.doi.org/10.1529/biophysj.107.128587
Ending Page	4560
Page Count	12
Starting Page	4549
File Format	PDF
ISSN	00063495
e-ISSN	15420086
Journal	Biophysical Journal
Issue Number	11
Volume Number	94
Language	English
Publisher	The Biophysical Society
Publisher Date	2008-06-01
Access Restriction	Open
Rights Holder	The Biophysical Society
Subject Keyword	Biophysics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics

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