NDLI: Isolation and Fractionation of the Photosynthetic Membranous Organelles from Rhodopseudomonas spheroides 1

Content Provider	PubMed Central
Author	Fraker, Pamela J. Kaplan, Samuel
Abstract	Molecular sieve chromatography and sucrose gradient centrifugation were used to prepare large quantities of purified chromatophores from Rhodopseudomonas spheroides. Electron micrographs of these chromatophores revealed that the final preparations were very homogeneous and free of non-chromatophore particulate material. As an additional check on purity, 14C-l-phenylalanine-labeled aerobic cells, devoid of chromatophores, were mixed with unlabeled photosynthetic cells. The resulting preparation contained less than 1% of the radioactivity, originally located in non-chromatophore protein. The purified chromatophores were solubilized in 2-chloroethanol and separated into two fractions. Fraction P1 contained 3 to 5% of the total chromatophore protein and could be resolved into 10 electrophoretic components. The second fraction, PII, contained five electrophoretic components. One of these components had associated with it all of the pigment and phospholipid present in PII. Preliminary immunochemical studies on these fractions are also reported.
Starting Page	465
File Format	PDF
ISSN	10985530
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	1
Volume Number	108
Language	English
Publisher Date	1971-10-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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Isolation and Fractionation of the Photosynthetic Membranous Organelles from Rhodopseudomonas spheroides 1