NDLI: Expression pattern and activity of six glutelin gene promoters in transgenic rice*

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Expression pattern and activity of six glutelin gene promoters in transgenic rice*

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Year: 2008, Volume: 59, Issue: 8

Year: 2007, Volume: 58

Expression pattern and activity of six glutelin gene promoters in transgenic rice*

Content Provider	PubMed Central
Author	Qu, Le Qing Xing, Yan Ping Liu, Wen Xian Xu, Xiu Ping Song, Yan Ru
Copyright Year	2008
Abstract	The shortage of strong endosperm-specific expression promoters for driving the expression of recombinant protein genes in cereal endosperm is a major limitation in obtaining the required level and pattern of expression. Six promoters of seed storage glutelin genes (GluA-1, GluA-2, GluA-3, GluB-3, GluB-5, and GluC) were isolated from rice (Oryza sativa L.) genomic DNA by PCR. Their spatial and temporal expression patterns and expression potential in stable transgenic rice plants were examined with β-glucuronidase (GUS) used as a reporter gene. All the promoters showed the expected spatial expression within the endosperm. The GluA-1, GluA-2, and GluA-3 promoters directed GUS expression mainly in the outer portion (peripheral region) of the endosperm. The GluB-5 and GluC promoters directed GUS expression in the whole endosperm, with the latter expressed almost evenly throughout the whole endosperm, a feature different from that of other rice glutelin gene promoters. The GluB-3 promoter directed GUS expression solely in aleurone and subaleurone layers. Promoter activities examined during seed maturation showed that the GluC promoter had much higher activity than the other promoters. These promoters are ideal candidates for achieving gene expression for multiple purposes in monocot endosperm but avoid promoter homology-based gene silencing. The GluC promoter did not contain the endosperm specificity-determining motifs GCN4, AACA, and the prolamin-box, which suggests the existence of additional regulatory mechanism in determining endosperm specificity.
Related Links	http://dx.doi.org/10.1093/jxb/ern110
Ending Page	2424
Page Count	8
Starting Page	2417
File Format	PDF
ISSN	00220957
e-ISSN	14602431
Journal	Journal of Experimental Botany
Issue Number	9
Volume Number	59
Language	English
Publisher	Oxford University Press
Publisher Date	2008-06-01
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Plant Science Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science

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