NDLI: Host range mutants of v-src: alterations in kinase activity and substrate interactions.

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Common and unique features of T antigens encoded by the polyomavirus group.

Purification and characterization of UL9, the herpes simplex virus type 1 origin-binding protein.

Posttranslational side chain modification of a viral epitope results in diminished recognition by specific T cells.

Expression and characterization of genetically engineered human immunodeficiency virus-like particles containing modified envelope glycoproteins: implications for development of a cross-protective AIDS vaccine.

Structural basis of C3b binding by glycoprotein C of herpes simplex virus.

Binding of the coronavirus mouse hepatitis virus A59 to its receptor expressed from a recombinant vaccinia virus depends on posttranslational processing of the receptor glycoprotein.

Detection of feline immunodeficiency virus (FIV) nucleic acids in FIV-seronegative cats.

Analysis of mutations in adeno-associated virus Rep protein in vivo and in vitro.

Vesicular stomatitis virus matrix protein inhibits host cell-directed transcription of target genes in vivo.

Human immunodeficiency virus type 1 Tat-mediated trans activation correlates with the phosphorylation state of a cellular TAR RNA stem-binding factor.

Transcriptional regulation of precore and pregenomic RNAs of hepatitis B virus.

The surface envelope protein gene region of equine infectious anemia virus is not an important determinant of tropism in vitro.

Induction of cytotoxic T cells to a cross-reactive epitope in the hepatitis C virus nonstructural RNA polymerase-like protein.

The arginine-rich domain of the hepatitis B virus core protein is required for pregenome encapsidation and productive viral positive-strand DNA synthesis but not for virus assembly.

Recombinant human adenoviruses containing hybrid adenovirus type 5 (Ad5)/Ad12 E1A genes: characterization of hybrid E1A proteins and analysis of transforming activity and host range.

Physical and functional interaction of human cytomegalovirus DNA polymerase and its accessory protein (ICP36) expressed in insect cells.

The transmembrane glycoprotein of human immunodeficiency virus type 1 induces syncytium formation in the absence of the receptor binding glycoprotein.

The human immunodeficiency virus type 1 packaging signal and major splice donor region have a conserved stable secondary structure.

Overexpression of the influenza virus polymerase can titrate out inhibition by the murine Mx1 protein.

Intracellular processing of the paramyxovirus F protein: critical role of the predicted amphipathic alpha helix adjacent to the fusion domain.

Role of cell-associated enveloped vaccinia virus in cell-to-cell spread.

Morphogenesis of Sindbis virus in three subclones of Aedes albopictus (mosquito) cells.

Induction of differentiation-associated changes in established human cells by infection with adeno-associated virus type 2.

Mutant p53 can substitute for human papillomavirus type 16 E6 in immortalization of human keratinocytes but does not have E6-associated trans-activation or transforming activity.

Mechanisms of interference with simian virus 40 (SV40) DNA replication by trans-dominant mutants of SV40 large T antigen.

Effect of retroviral proteinase inhibitors on Mason-Pfizer monkey virus maturation and transmembrane glycoprotein cleavage.

Transcellular activation of the human immunodeficiency virus type 1 long terminal repeat in cocultured lymphocytes.

Association of the nonstructural protein NSs of Uukuniemi virus with the 40S ribosomal subunit.

Two distant upstream regions containing cis-acting signals regulating splicing facilitate 3'-end processing of avian sarcoma virus RNA.

Mechanisms of antibody-mediated protection against lymphocytic choriomeningitis virus infection: mother-to-baby transfer of humoral protection.

Mutational analysis of the N-linked glycosylation sites of the SU envelope protein of Moloney murine leukemia virus.

Identification of envelope protein epitopes that are important in the attenuation process of wild-type yellow fever virus.

When retroviral reverse transcriptases reach the end of their RNA templates.

Inhibition of virus production in peripheral blood mononuclear cells from human immunodeficiency virus (HIV) type 1-seropositive donors by treatment with recombinant HIV-like particles.

Comparative analysis of Rev function in human immunodeficiency virus types 1 and 2.

UL34, the target of the herpes simplex virus U(S)3 protein kinase, is a membrane protein which in its unphosphorylated state associates with novel phosphoproteins.

Transactivation of the major capsid protein gene of herpes simplex virus type 1 requires a cellular transcription factor.

Host range mutants of v-src: alterations in kinase activity and substrate interactions.

Phosphorylation regulates RNA binding by the human T-cell leukemia virus Rex protein.

Mutational analysis of the promoter required for influenza virus virion RNA synthesis.

Generation and analysis of defective genomes of Autographa californica nuclear polyhedrosis virus.

Multistep regulation of enhancer activity of the 21-base-pair element of human T-cell leukemia virus type I.

Two distinct serum mannose-binding lectins function as beta inhibitors of influenza virus: identification of bovine serum beta inhibitor as conglutinin.

Conservation of RNA-protein interactions among picornaviruses.

Enhanced gene expression of the murine ecotropic retroviral receptor and its human homolog in proliferating cells.

Alternate translation initiation on hepatitis B virus X mRNA produces multiple polypeptides that differentially transactivate class II and III promoters.

Expression of the Marek's disease virus homolog of herpes simplex virus glycoprotein B in Escherichia coli and its identification as B antigen.

Identification of the murine cytomegalovirus glycoprotein B gene and its expression by recombinant vaccinia virus.

Expression of the OSU rotavirus outer capsid protein VP4 by an adenovirus recombinant.

Vaccinia virus B1 kinase: phenotypic analysis of temperature-sensitive mutants and enzymatic characterization of recombinant proteins.

Inhibition of protein kinase C results in decreased expression of bovine leukemia virus.

Human cytomegalovirus contains a tegument protein that enhances transcription from promoters with upstream ATF and AP-1 cis-acting elements.

Changes in both gp120 and gp41 can account for increased growth potential and expanded host range of human immunodeficiency virus type 1.

The human cytomegalovirus 80-kilodalton but not the 72-kilodalton immediate-early protein transactivates heterologous promoters in a TATA box-dependent mechanism and interacts directly with TFIID.

Neutralization of multiple laboratory and clinical isolates of human immunodeficiency virus type 1 (HIV-1) by antisera raised against gp120 from the MN isolate of HIV-1.

Constitutive expression of an ISGF2/IRF1 transgene leads to interferon-independent activation of interferon-inducible genes and resistance to virus infection.

Matrix protein of Akv murine leukemia virus: genetic mapping of regions essential for particle formation.

Premature termination and processing of human immunodeficiency virus type 1-promoted transcripts.

Protection against Friend retrovirus-induced leukemia by recombinant vaccinia viruses expressing the gag gene.

Influence of Theiler's murine encephalomyelitis virus 5' untranslated region on translation and neurovirulence.

Persistent infection of rabbits with bovine immunodeficiency-like virus.

Localization to the Golgi complex of Uukuniemi virus glycoproteins G1 and G2 expressed from cloned cDNAs.

Role of the fusogenic peptide sequence in syncytium induction and infectivity of human immunodeficiency virus type 2.

Transcellular activation of the human immunodeficiency virus type 1 long terminal repeat in T lymphocytes requires CD4-gp120 binding.

Dominant-negative mutants are clustered in a domain of the human T-cell leukemia virus type I Rex protein: implications for trans dominance.

In vivo genetic variability of the human immunodeficiency virus type 2 V3 region.

Phosphorylation of the budgerigar fledgling disease virus major capsid protein VP1.

Myristylation of poliovirus capsid precursor P1 is required for assembly of subviral particles.

Biological activity of paramyxovirus fusion proteins: factors influencing formation of syncytia.

Role of human T-cell leukemia virus type 1 X region proteins in immortalization of primary human lymphocytes in culture.

Simian virus 40 large T-antigen function is required for induction of tetraploid DNA content during lytic infection.

Expression pattern of mouse mammary tumor virus in transgenic mice carrying exogenous proviruses of different origins.

Infection by polyomavirus of murine cells deficient in class I major histocompatibility complex expression.

Activation of simian virus 40 transcription in vitro by T antigen.

Molecular basis of antigenic structures of poliovirus: implications for their evolution during morphogenesis.

Lymphocyte-facilitated infection of epithelia by human T-cell lymphotropic virus type I.

Adenovirus E1A makes two distinct contacts with the retinoblastoma protein.

Phenotypes of Epstein-Barr virus LMP1 deletion mutants indicate transmembrane and amino-terminal cytoplasmic domains necessary for effects in B-lymphoma cells.

Human chromosome 12 is required for optimal interactions between Tat and TAR of human immunodeficiency virus type 1 in rodent cells.

Evolution of the V3 envelope domain in proviral sequences and isolates of human immunodeficiency virus type 1 during transition of the viral biological phenotype.

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Host range mutants of v-src: alterations in kinase activity and substrate interactions.

Content Provider	PubMed Central
Author	Liebl, E. C. Declue, J. E. Martin, G. S. England, L. J.
Abstract	Host range mutants of Schmidt-Ruppin v-src that transform chicken embryo fibroblasts (CEF) but not Rat-2 cells were generated previously by linker insertion-deletion mutagenesis (J. E. DeClue and G. S. Martin, J. Virol. 63:542-554, 1989). One of these mutants, SRX5, in which Tyr-416 is substituted by the sequence Ser Arg Asp, retained high levels of kinase activity in vitro and in vivo, both in CEF and in Rat-2 cells. Phosphorylation of p36 (the calpactin I heavy chain) was drastically reduced in cells expressing SRX5 src, suggesting that the phenotype of SRX5 results from an alteration in substrate recognition by the src kinase. Three mutants, SPX1, SHX13, and XD6, containing linker insertions or small deletions within the src homology 2 (SH2) region, induced reduced levels of kinase activity in both CEF and Rat-2 cells. However, the residual levels of kinase activity in Rat-2 cells were above the threshold at which wild-type pp60v-src transforms Rat-2 cells, indicating that the reduction in kinase activity was not sufficient to account for the failure to transform. Cells infected by these mutants exhibited reduced levels of phosphorylation of 120- and 62-kDa proteins. We have reported elsewhere (M. F. Moran, C. A. Koch, D. Anderson, C. Ellis, L. England, G. S. Martin, and T. Pawson, Proc. Natl. Acad. Sci. USA 87:8622-8626, 1990) that ras GTPase-activating protein GAP and associated protein p62 are not tyrosine phosphorylated in Rat-2 cells expressing SHX13 or XD6. The transformation defect in Rat-2 cells may result from the failure to phosphorylate those proteins. The fifth mutant, XD4, contains a deletion which removes all of the src homology 3 (SH3) and most of the SH2 sequences of src. The protein encoded by XD4 is active as a kinase when expressed in CEF, indicating that in CEF the SH2 and SH3 regions of v-src are not necessary for kinase activity and transformation. The XD4 src product is not tyrosine phosphorylated and is inactive as a kinase when expressed in Rat-2 cells. Thus, host cell factors can affect the tyrosine phosphorylation and activity of the v-src kinase in the absence of the SH2 and SH3 regions. These results indicate that the host-dependent transformation phenotype results from alterations in src kinase activity and substrate specificity.
Starting Page	4315
File Format	PDF
ISSN	10985514
e-ISSN	10985514
Journal	Journal of Virology
Issue Number	7
Volume Number	66
Language	English
Publisher Date	1992-07-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology Insect Science

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