NDLI: Selection of mammalian cells based on their cell-cycle phase using dielectrophoresis

Content Provider	PubMed Central
Author	Kim, Unyoung Shu, Chih-wen Dane, Karen Y. Daugherty, Patrick S. Wang, Jean Y. J. Soh, H. T.
Copyright Year	2007
Abstract	An effective, noninvasive means of selecting cells based on their phase within the cell cycle is an important capability for biological research. Current methods of producing synchronous cell populations, however, tend to disrupt the natural physiology of the cell or suffer from low synchronization yields. In this work, we report a microfluidic device that utilizes the dielectrophoresis phenomenon to synchronize cells by exploiting the relationship between the cell's volume and its phase in the cell cycle. The dielectrophoresis activated cell synchronizer (DACSync) device accepts an asynchronous mixture of cells at the inlet, fractionates the cell populations according to the cell-cycle phase (G1/S and G2/M), and elutes them through different outlets. The device is gentle and efficient; it utilizes electric fields that are 1–2 orders of magnitude below those used in electroporation and enriches asynchronous tumor cells in the G1 phase to 96% in one round of sorting, in a continuous flow manner at a throughput of 2 × 105 cells per hour per microchannel. This work illustrates the feasibility of using laminar flow and electrokinetic forces for the efficient, noninvasive separation of living cells.
Related Links	http://dx.doi.org/10.1073/pnas.0708760104
Ending Page	20712
Page Count	5
Starting Page	20708
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	52
Volume Number	104
Language	English
Publisher	National Academy of Sciences
Publisher Date	2007-12-26
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Selection of mammalian cells based on their cell-cycle phase using dielectrophoresis.

Marker-specific sorting of rare cells using dielectrophoresis

Cell Cycle-Dependent Microtubule-Based Dynamic Transport of Cytoplasmic Dynein in Mammalian Cells

Phase locking and multiple oscillating attractors for the coupled mammalian clock and cell cycle

Regulation of Kv1 subunit expression in oligodendrocyte progenitor cells and their role in G1/S phase progression of the cell cycle

Transformed mammalian cells are deficient in kinase-mediated control of progression through the G1 phase of the cell cycle.

Auditory ganglion source of Sonic hedgehog regulates timing of cell cycle exit and differentiation of mammalian cochlear hair cells

Selection of Metastatic Breast Cancer Cells Based on Adaptability of Their Metabolic State

Cell-cycle-specific cDNAs from mammalian cells temperature sensitive for growth.

Selection of mammalian cells based on their cell-cycle phase using dielectrophoresis

Similar Documents

Selection of mammalian cells based on their cell-cycle phase using dielectrophoresis.

Marker-specific sorting of rare cells using dielectrophoresis

Cell Cycle-Dependent Microtubule-Based Dynamic Transport of Cytoplasmic Dynein in Mammalian Cells

Phase locking and multiple oscillating attractors for the coupled mammalian clock and cell cycle

Regulation of Kv1 subunit expression in oligodendrocyte progenitor cells and their role in G1/S phase progression of the cell cycle

Transformed mammalian cells are deficient in kinase-mediated control of progression through the G1 phase of the cell cycle.

Auditory ganglion source of Sonic hedgehog regulates timing of cell cycle exit and differentiation of mammalian cochlear hair cells

Selection of Metastatic Breast Cancer Cells Based on Adaptability of Their Metabolic State

Cell-cycle-specific cDNAs from mammalian cells temperature sensitive for growth.

Selection of mammalian cells based on their cell-cycle phase using dielectrophoresis