NDLI: Identification of the catalytic sites of a papain-like cysteine proteinase of murine coronavirus.

Please wait, while we are loading the content...

Year: 2016, Volume: 90

Year: 2015, Volume: 90

Year: 2015, Volume: 89

Year: 2014, Volume: 89

Year: 2014, Volume: 88

Year: 2013, Volume: 87

Year: 2012, Volume: 86

Year: 2011, Volume: 85

Year: 2010, Volume: 84

Year: 2009, Volume: 83

Year: 2008, Volume: 82

Year: 2007, Volume: 81

Year: 2006, Volume: 80

Year: 2005, Volume: 79

Year: 2004, Volume: 78

Year: 2003, Volume: 77

Year: 2002, Volume: 76

Year: 2001, Volume: 75

Year: 2000, Volume: 74

Year: 1999, Volume: 73

Year: 1998, Volume: 72

Year: 1997, Volume: 71

Year: 1996, Volume: 70

Year: 1995, Volume: 69

Year: 1994, Volume: 68

Year: 1993, Volume: 67

Year: 1993, Volume: 67, Issue: 12

Year: 1993, Volume: 67, Issue: 11

Year: 1993, Volume: 67, Issue: 10

Mechanisms of immune activation of human immunodeficiency virus in monocytes/macrophages.

Dissociation of influenza virus hemagglutinin and neuraminidase eliminates their intravirionic antigenic competition.

Delayed cytopathicity of a feline leukemia virus variant is due to four mutations in the transmembrane protein gene.

Long-range mapping of Mis-2, a common provirus integration site identified in murine leukemia virus-induced thymomas and located 160 kilobase pairs downstream of Myb.

Mutational analysis of a predicted zinc-binding motif in the 26-kilodalton protein encoded by the vaccinia virus A2L gene: correlation of zinc binding with late transcriptional transactivation activity.

Programmed cell death of T lymphocytes during acute viral infection: a mechanism for virus-induced immune deficiency.

Mutation of large T-antigen-binding site A, but not site B or C, eliminates stalling by RNA polymerase II in the intergenic region of polyomavirus DNA.

The hr5 transcriptional enhancer stimulates early expression from the Autographa californica nuclear polyhedrosis virus genome but is not required for virus replication.

Severity of neurological signs and degree of inflammatory lesions in the brains of rats with Borna disease correlate with the induction of nitric oxide synthase.

Nuclear organization of splicing small nuclear ribonucleoproteins in adenovirus-infected cells.

The conserved N-terminal region of Sendai virus nucleocapsid protein NP is required for nucleocapsid assembly.

Autoproteolysis of herpes simplex virus type 1 protease releases an active catalytic domain found in intermediate capsid particles.

Suppression of hepatitis B virus expression and replication by hepatitis C virus core protein in HuH-7 cells.

Alternative transcriptional initiation as a novel mechanism for regulating expression of a baculovirus trans activator.

Influence of epitope polarity and adjuvants on the immunogenicity and efficacy of a synthetic peptide vaccine against Semliki Forest virus.

Rubella virus-specific cytotoxic T-lymphocyte responses: identification of the capsid as a target of major histocompatibility complex class I-restricted lysis and definition of two epitopes.

The 1,629-nucleotide open reading frame located downstream of the Autographa californica nuclear polyhedrosis virus polyhedrin gene encodes a nucleocapsid-associated phosphoprotein.

Sequence and temporal appearance of the early transcribed baculovirus gene HE65.

Infection of peripancreatic lymph nodes but not islets precedes Kilham rat virus-induced diabetes in BB/Wor rats.

Differential replication of human immunodeficiency virus type 1 in CD8- and CD8+ subsets of natural killer cells: relationship to cytokine production pattern.

The feline immunodeficiency virus ORF-A gene facilitates efficient viral replication in established T-cell lines and peripheral blood lymphocytes.

Genetic variability and evolution of the satellite RNA of cucumber mosaic virus during natural epidemics.

Possible involvement of microtubule disruption in bipolar budding of a Sendai virus mutant, F1-R, in epithelial MDCK cells.

Packaging capacity and stability of human adenovirus type 5 vectors.

Two regions of the herpes simplex virus type 1 UL42 protein are required for its functional interaction with the viral DNA polymerase.

Effects of temperature and lipophilic agents on poliovirus formation and RNA synthesis in a cell-free system.

Cofactor requirement for human immunodeficiency virus type 1 entry into a CD4-expressing human cell line.

Glycoprotein B is a specific determinant of herpes simplex virus type 1 neuroinvasiveness.

A 3' coterminal gene cluster in pseudorabies virus contains herpes simplex virus UL1, UL2, and UL3 gene homologs and a unique UL3.5 open reading frame.

Dengue virus-specific human CD4+ T-lymphocyte responses in a recipient of an experimental live-attenuated dengue virus type 1 vaccine: bulk culture proliferation, clonal analysis, and precursor frequency determination.

Spontaneous mutagenesis of a plant potyvirus genome after insertion of a foreign gene.

Characterization of chimeric full-length molecular clones of Aleutian mink disease parvovirus (ADV): identification of a determinant governing replication of ADV in cell culture.

The MA (p15) and p12 regions of the gag gene are sufficient for the pathogenicity of the murine AIDS virus.

DNA-binding domain of bovine papillomavirus type 1 E1 helicase: structural and functional aspects.

Intracerebral hemorrhages and syncytium formation induced by endothelial cell infection with a murine leukemia virus.

Human membrane cofactor protein (CD46) acts as a cellular receptor for measles virus.

BCR-ABL and v-abl oncogenes induce distinct patterns of thymic lymphoma involving different lymphocyte subsets.

Viral determinants of simian immunodeficiency virus (SIV) virulence in rhesus macaques assessed by using attenuated and pathogenic molecular clones of SIVmac.

Identification of the catalytic sites of a papain-like cysteine proteinase of murine coronavirus.

Chimeric hepatitis B virus core particles with parts or copies of the hepatitis C virus core protein.

Distinct repertoire of antigenic variants of foot-and-mouth disease virus in the presence or absence of immune selection.

Rabies virus antinucleoprotein antibody protects against rabies virus challenge in vivo and inhibits rabies virus replication in vitro.

Features of the adeno-associated virus origin involved in substrate recognition by the viral Rep protein.

Transplacental transmission of a leukemogenic murine leukemia virus.

Deletion mapping of a mouse hepatitis virus defective interfering RNA reveals the requirement of an internal and discontiguous sequence for replication.

Lymphocytes protect against and are not required for reovirus-induced myocarditis.

The RR1 gene of herpes simplex virus type 1 is uniquely trans activated by ICP0 during infection.

Probing the structure of the V2 domain of human immunodeficiency virus type 1 surface glycoprotein gp120 with a panel of eight monoclonal antibodies: human immune response to the V1 and V2 domains.

Hepatitis C virus NS3 protein polynucleotide-stimulated nucleoside triphosphatase and comparison with the related pestivirus and flavivirus enzymes.

Mapping of functionally important residues of a cysteine-histidine box in the human immunodeficiency virus type 1 nucleocapsid protein.

The human papillomavirus type 6 and 16 E5 proteins are membrane-associated proteins which associate with the 16-kilodalton pore-forming protein.

Strain specificity and binding affinity requirements of neutralizing monoclonal antibodies to the C4 domain of gp120 from human immunodeficiency virus type 1.

Binding of nuclear factors to functional domains of the duck hepatitis B virus enhancer.

Identification and mapping of dimerization and DNA-binding domains in the C terminus of the IE2 regulatory protein of human cytomegalovirus.

trans complementation of cap-independent translation directed by poliovirus 5' noncoding region deletion mutants: evidence for RNA-RNA interactions.

In vitro and in vivo binding of human immunodeficiency virus type 1 Tat protein and Sp1 transcription factor.

Host range expansion of Autographa californica nuclear polyhedrosis virus (NPV) following recombination of a 0.6-kilobase-pair DNA fragment originating from Bombyx mori NPV.

Inhibition of Bombyx mori nuclear polyhedrosis virus (NPV) replication by the putative DNA helicase gene of Autographa californica NPV.

Necessity of the spacer peptide between CA and NC in the Rous sarcoma virus gag protein.

Amino acid changes in the fourth conserved region of human immunodeficiency virus type 2 strain HIV-2ROD envelope glycoprotein modulate fusion.

Specificity analysis of human CD4+ T-cell clones directed against human herpesvirus 6 (HHV-6), HHV-7, and human cytomegalovirus.

Influence of sequences in the long terminal repeat and flanking cell DNA on polyadenylation of retroviral transcripts.

Congo red inhibition of scrapie agent replication.

Two cis-acting signals control ribosomal frameshift between human T-cell leukemia virus type II gag and pro genes.

Importance of the bovine papillomavirus P2443 promoter in the regulation of E2 and E5 expression.

Definition of an HLA-DPw2-restricted epitope on NS3, recognized by a dengue virus serotype-cross-reactive human CD4+ CD8- cytotoxic T-cell clone.

Role of the cytoplasmic domains of viral glycoproteins in antibody-induced cell surface mobility.

Cytopathogenic effect in cardiac myocytes but not in cardiac fibroblasts is correlated with reovirus-induced acute myocarditis.

Divergence between cytotoxic effector function and tumor necrosis factor alpha production for inflammatory CD4+ T cells from mice with Sendai virus pneumonia.

Epstein-Barr virus (EBV) gene expression in EBV-positive peripheral T-cell lymphomas.

Functional and genetic plasticities of the poliovirus genome: quasi-infectious RNAs modified in the 5'-untranslated region yield a variety of pseudorevertants.

Phenotypic and functional consequences of herpesvirus saimiri infection of human CD8+ cytotoxic T lymphocytes.

Characterization of the DNA binding properties of polyomavirus capsid protein.

Epitopes of glycoprotein G of equine herpesviruses 4 and 1 located near the C termini elicit type-specific antibody responses in the natural host.

Year: 1993, Volume: 67, Issue: 9

Year: 1993, Volume: 67, Issue: 8

Year: 1993, Volume: 67, Issue: 7

Year: 1993, Volume: 67, Issue: 6

Year: 1993, Volume: 67, Issue: 5

Year: 1993, Volume: 67, Issue: 4

Year: 1993, Volume: 67, Issue: 3

Year: 1993, Volume: 67, Issue: 2

Year: 1993, Volume: 67, Issue: 1

Year: 1992, Volume: 66

Year: 1991, Volume: 65

Year: 1990, Volume: 64

Year: 1989, Volume: 63

Year: 1988, Volume: 62

Year: 1987, Volume: 61

Year: 1986, Volume: 60

Year: 1986, Volume: 59

Year: 1986, Volume: 58

Year: 1986, Volume: 57

Year: 1985, Volume: 56

Year: 1985, Volume: 55

Year: 1985, Volume: 54

Year: 1985, Volume: 53

Year: 1984, Volume: 52

Year: 1984, Volume: 51

Year: 1984, Volume: 50

Year: 1984, Volume: 49

Year: 1983, Volume: 48

Year: 1983, Volume: 47

Year: 1983, Volume: 46

Year: 1983, Volume: 45

Year: 1982, Volume: 44

Year: 1982, Volume: 43

Year: 1982, Volume: 42

Year: 1982, Volume: 41

Year: 1981, Volume: 40

Year: 1981, Volume: 39

Year: 1981, Volume: 38

Year: 1981, Volume: 37

Year: 1980, Volume: 36

Year: 1980, Volume: 35

Year: 1980, Volume: 34

Year: 1980, Volume: 33

Year: 1979, Volume: 32

Year: 1979, Volume: 31

Year: 1979, Volume: 30

Year: 1979, Volume: 29

Year: 1978, Volume: 28

Year: 1978, Volume: 27

Year: 1978, Volume: 26

Year: 1978, Volume: 25

Year: 1977, Volume: 24

Year: 1977, Volume: 23

Year: 1977, Volume: 22

Year: 1977, Volume: 21

Year: 1976, Volume: 20

Year: 1976, Volume: 19

Year: 1976, Volume: 18

Year: 1976, Volume: 17

Year: 1975, Volume: 16

Year: 1975, Volume: 15

Year: 1974, Volume: 14

Year: 1974, Volume: 13

Year: 1973, Volume: 12

Year: 1973, Volume: 11

Year: 1972, Volume: 10

Year: 1972, Volume: 9

Year: 1971, Volume: 8

Year: 1971, Volume: 7

Year: 1970, Volume: 6

Year: 1970, Volume: 5

Year: 1969, Volume: 4

Year: 1969, Volume: 3

Year: 1968, Volume: 2

Year: 1967, Volume: 1

Identification of the catalytic sites of a papain-like cysteine proteinase of murine coronavirus.

Content Provider	PubMed Central
Author	Baker, S. C. Yokomori, K. Dong, S. Carlisle, R. Gorbalenya, A. E. Lai, M. M. Koonin, E. V.
Abstract	The murine coronavirus mouse hepatitis virus gene 1 is expressed as a polyprotein, which is cleaved into multiple proteins posttranslationally. One of the proteins is p28, which represents the amino-terminal portion of the polyprotein and is presumably generated by the activity of an autoproteinase domain of the polyprotein (S. C. Baker, C. K. Shieh, L. H. Soe, M.-F. Chang, D. M. Vannier, and M. M. C. Lai, J. Virol. 63:3693-3699, 1989). In this study, the boundaries and the critical amino acid residues of this putative proteinase domain were characterized by deletion analysis and site-directed mutagenesis. Proteinase activity was monitored by examining the generation of p28 during in vitro translation in rabbit reticulocyte lysates. Deletion analysis defined the proteinase domain to be within the sequences encoded from the 3.6- to 4.4-kb region from the 5' end of the genome. A 0.7-kb region between the substrate (p28) and proteinase domain could be deleted without affecting the proteolytic cleavage. However, a larger deletion (1.6 kb) resulted in the loss of proteinase activity, suggesting the importance of spacing sequences between proteinase and substrate. Computer-assisted analysis of the amino acid sequence of the proteinase domain identified potential catalytic cysteine and histidine residues in a stretch of sequence distantly related to papain-like cysteine proteinases. The role of these putative catalytic residues in the proteinase activity was studied by site-specific mutagenesis. Mutations of Cys-1137 or His-1288 led to a complete loss of proteinase activity, implicating these residues as essential for the catalytic activity. In contrast, most mutations of His-1317 or Cys-1172 had no or only minor effects on proteinase activity. This study establishes that mouse hepatitis virus gene 1 encodes a proteinase domain, in the region from 3.6 to 4.4 kb from the 5' end of the genome, which resembles members of the papain family of cysteine proteinases and that this proteinase domain is responsible for the cleavage of the N-terminal peptide.
Starting Page	6056
File Format	PDF
ISSN	10985514
e-ISSN	10985514
Journal	Journal of Virology
Issue Number	10
Volume Number	67
Language	English
Publisher Date	1993-10-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology Insect Science

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in