NDLI: Functional dissection of the human spumaretrovirus transactivator identifies distinct classes of dominant-negative mutants.

Please wait, while we are loading the content...

Year: 2016, Volume: 90

Year: 2015, Volume: 90

Year: 2015, Volume: 89

Year: 2014, Volume: 89

Year: 2014, Volume: 88

Year: 2013, Volume: 87

Year: 2012, Volume: 86

Year: 2011, Volume: 85

Year: 2010, Volume: 84

Year: 2009, Volume: 83

Year: 2008, Volume: 82

Year: 2007, Volume: 81

Year: 2006, Volume: 80

Year: 2005, Volume: 79

Year: 2004, Volume: 78

Year: 2003, Volume: 77

Year: 2002, Volume: 76

Year: 2001, Volume: 75

Year: 2000, Volume: 74

Year: 1999, Volume: 73

Year: 1998, Volume: 72

Year: 1997, Volume: 71

Year: 1996, Volume: 70

Year: 1995, Volume: 69

Year: 1994, Volume: 68

Year: 1993, Volume: 67

Year: 1993, Volume: 67, Issue: 12

Year: 1993, Volume: 67, Issue: 11

Year: 1993, Volume: 67, Issue: 10

Year: 1993, Volume: 67, Issue: 9

Year: 1993, Volume: 67, Issue: 8

Year: 1993, Volume: 67, Issue: 7

Year: 1993, Volume: 67, Issue: 6

Year: 1993, Volume: 67, Issue: 5

Year: 1993, Volume: 67, Issue: 4

Year: 1993, Volume: 67, Issue: 3

Year: 1993, Volume: 67, Issue: 2

Year: 1993, Volume: 67, Issue: 1

Several members of the mouse carcinoembryonic antigen-related glycoprotein family are functional receptors for the coronavirus mouse hepatitis virus-A59.

Modulating effects of the extracellular sequence of the human insulinlike growth factor I receptor on its transforming and tumorigenic potential.

Proteolytic processing of Ty3 proteins is required for transposition.

Bidirectional entry of poliovirus into polarized epithelial cells.

Identification of alternatively spliced mRNAs encoding potential new regulatory proteins in cattle infected with bovine leukemia virus.

Complement activation by human monoclonal antibodies to human immunodeficiency virus.

Cell fusion induced by the murine leukemia virus envelope glycoprotein.

Susceptibility to measles virus-induced encephalitis in mice correlates with impaired antigen presentation to cytotoxic T lymphocytes.

Herpes simplex virus infection and propagation in a mouse L cell mutant lacking heparan sulfate proteoglycans.

Pathogenesis of adenovirus type 5 pneumonia in cotton rats (Sigmodon hispidus).

Mouse mammary tumor virus with rearranged long terminal repeats causes murine lymphomas.

Role of nuclear pore complex in simian virus 40 nuclear targeting.

The minor capsid protein VP1 of the autonomous parvovirus minute virus of mice is dispensable for encapsidation of progeny single-stranded DNA but is required for infectivity.

Differentiated liver cell specificity of the second enhancer of hepatitis B virus.

Functional role of the glycan cluster of the human immunodeficiency virus type 1 transmembrane glycoprotein (gp41) ectodomain.

Functional dissection of the human spumaretrovirus transactivator identifies distinct classes of dominant-negative mutants.

Envelope glycoprotein gp50 of pseudorabies virus is essential for virus entry but is not required for viral spread in mice.

Avian retroviral RNA encapsidation: reexamination of functional 5' RNA sequences and the role of nucleocapsid Cys-His motifs.

Incorporation of CD4 into virions by a recombinant herpes simplex virus.

Characterization of ribosomal frameshifting for expression of pol gene products of human T-cell leukemia virus type I.

DNA strand exchange catalyzed by proteins from vaccinia virus-infected cells.

Mutations in the cytoplasmic domain of human immunodeficiency virus type 1 transmembrane protein impair the incorporation of Env proteins into mature virions.

Genetic bottlenecks and population passages cause profound fitness differences in RNA viruses.

Expression of Aleutian mink disease parvovirus proteins in a baculovirus vector system.

TAR-independent transactivation of the murine cytomegalovirus major immediate-early promoter by the Tat protein.

A micromolar pool of antigenically distinct precursors is required to initiate cooperative assembly of hepatitis B virus capsids in Xenopus oocytes.

Inhibition of herpes simplex virus type 1 DNA polymerase activity by peptides from the UL42 accessory protein is largely nonspecific.

Analysis of the adenovirus type 5 terminal protein precursor and DNA polymerase by linker insertion mutagenesis.

Release, uptake, and effects of extracellular human immunodeficiency virus type 1 Tat protein on cell growth and viral transactivation.

Mutational analysis of the p50 subunit of NF-kappa B and inhibition of NF-kappa B activity by trans-dominant p50 mutants.

An increase in disease latency is associated with a host-dependent selection for recombinant murine leukemia viruses with substitutions in the p15E (TM) gene.

Varicella-zoster virus glycoprotein gpI/gpIV receptor: expression, complex formation, and antigenicity within the vaccinia virus-T7 RNA polymerase transfection system.

Self-assembly of human papillomavirus type 1 capsids by expression of the L1 protein alone or by coexpression of the L1 and L2 capsid proteins.

The 86-kilodalton IE-2 protein of human cytomegalovirus is a sequence-specific DNA-binding protein that interacts directly with the negative autoregulatory response element located near the cap site of the IE-1/2 enhancer-promoter.

A "string-of-beads" vaccine, comprising linked minigenes, confers protection from lethal-dose virus challenge.

A single point mutation in the VP7 major core protein of bluetongue virus prevents the formation of core-like particles.

Cytoplasmic domain requirement for incorporation of a foreign envelope protein into vesicular stomatitis virus.

Role of the large hepatitis B virus envelope protein in infectivity of the hepatitis delta virion.

Enzymatic activity of poliovirus RNA polymerases with mutations at the tyrosine residue of the conserved YGDD motif: isolation and characterization of polioviruses containing RNA polymerases with FGDD and MGDD sequences.

Progression from papilloma to carcinoma is accompanied by changes in antibody response to papillomavirus proteins.

Formation of rhinovirus-soluble ICAM-1 complexes and conformational changes in the virion.

The extreme C terminus of herpes simplex virus DNA polymerase is crucial for functional interaction with processivity factor UL42 and for viral replication.

Membrane association of functional vesicular stomatitis virus matrix protein in vivo.

A mouse mammary tumor virus promoter element near the transcription initiation site.

Characterization of human immunodeficiency virus type 1 integrase expressed in Escherichia coli and analysis of variants with amino-terminal mutations.

Recognition of a highly conserved region of human immunodeficiency virus type 1 gp120 by an HLA-Cw4-restricted cytotoxic T-lymphocyte clone.

Hepatitis delta virus antigen forms dimers and multimeric complexes in vivo.

Identification and characterization of the p35 gene of Bombyx mori nuclear polyhedrosis virus that prevents virus-induced apoptosis.

Immunodominance of the VP4 neutralization protein of rotavirus in protective natural infections of young children.

A 37-kilodalton glycoprotein from a baculovirus of Orgyia pseudotsugata is localized to cytoplasmic inclusion bodies.

Identification of specific adenovirus E1A N-terminal residues critical to the binding of cellular proteins and to the control of cell growth.

Neutralizing monoclonal antibodies that distinguish three antigenic sites on human cytomegalovirus glycoprotein H have conformationally distinct binding sites.

Expression and analysis of the human cytomegalovirus UL80-encoded protease: identification of autoproteolytic sites.

The adenovirus E1A 12S product displays functional redundancy in activating the human proliferating cell nuclear antigen promoter.

Identification of a large bent DNA domain and binding sites for serum response factor adjacent to the NFI repeat cluster and enhancer region in the major IE94 promoter from simian cytomegalovirus.

Evidence from the anti-idiotypic network that the acetylcholine receptor is a rabies virus receptor.

Mutations in the C terminus of herpes simplex virus type 1 DNA polymerase can affect binding and stimulation by its accessory protein UL42 without affecting basal polymerase activity.

Borna disease virus in mice: host-specific differences in disease expression.

The cytoplasmic domain of the human T-cell leukemia virus type I envelope can modulate envelope functions in a cell type-dependent manner.

Infectious virus replication in papillomas induced by molecularly cloned cottontail rabbit papillomavirus DNA.

Human JC virus perfect palindromic nuclear factor 1-binding sequences important for glial cell-specific expression in differentiating embryonal carcinoma cells.

Immune response of rhesus macaques to recombinant simian immunodeficiency virus gp130 does not protect from challenge infection.

Glycosylation is necessary for the correct folding of human immunodeficiency virus gp120 in CD4 binding.

Theiler's virus infection of beta 2-microglobulin-deficient mice.

A mutant CHO-K1 strain with resistance to Pseudomonas exotoxin A is unable to process the precursor fusion glycoprotein of Newcastle disease virus.

Multipartite nature of potato virus X.

Experimental vaccine protection against homologous and heterologous strains of feline immunodeficiency virus.

Marker rescue of a transformation-negative Epstein-Barr virus recombinant from an infected Burkitt lymphoma cell line: a method useful for analysis of genes essential for transformation.

Inversion of a chicken ets-1 proto-oncogene segment in avian leukemia virus E26.

Recognition by and in vitro induction of cytotoxic T lymphocytes against predicted epitopes of the immediate-early protein ICP27 of herpes simplex virus.

Cap-Independent Translation by the 5′ Untranslated Region of Theiler's Murine Encephalomyelitis Virus

Year: 1992, Volume: 66

Year: 1991, Volume: 65

Year: 1990, Volume: 64

Year: 1989, Volume: 63

Year: 1988, Volume: 62

Year: 1987, Volume: 61

Year: 1986, Volume: 60

Year: 1986, Volume: 59

Year: 1986, Volume: 58

Year: 1986, Volume: 57

Year: 1985, Volume: 56

Year: 1985, Volume: 55

Year: 1985, Volume: 54

Year: 1985, Volume: 53

Year: 1984, Volume: 52

Year: 1984, Volume: 51

Year: 1984, Volume: 50

Year: 1984, Volume: 49

Year: 1983, Volume: 48

Year: 1983, Volume: 47

Year: 1983, Volume: 46

Year: 1983, Volume: 45

Year: 1982, Volume: 44

Year: 1982, Volume: 43

Year: 1982, Volume: 42

Year: 1982, Volume: 41

Year: 1981, Volume: 40

Year: 1981, Volume: 39

Year: 1981, Volume: 38

Year: 1981, Volume: 37

Year: 1980, Volume: 36

Year: 1980, Volume: 35

Year: 1980, Volume: 34

Year: 1980, Volume: 33

Year: 1979, Volume: 32

Year: 1979, Volume: 31

Year: 1979, Volume: 30

Year: 1979, Volume: 29

Year: 1978, Volume: 28

Year: 1978, Volume: 27

Year: 1978, Volume: 26

Year: 1978, Volume: 25

Year: 1977, Volume: 24

Year: 1977, Volume: 23

Year: 1977, Volume: 22

Year: 1977, Volume: 21

Year: 1976, Volume: 20

Year: 1976, Volume: 19

Year: 1976, Volume: 18

Year: 1976, Volume: 17

Year: 1975, Volume: 16

Year: 1975, Volume: 15

Year: 1974, Volume: 14

Year: 1974, Volume: 13

Year: 1973, Volume: 12

Year: 1973, Volume: 11

Year: 1972, Volume: 10

Year: 1972, Volume: 9

Year: 1971, Volume: 8

Year: 1971, Volume: 7

Year: 1970, Volume: 6

Year: 1970, Volume: 5

Year: 1969, Volume: 4

Year: 1969, Volume: 3

Year: 1968, Volume: 2

Year: 1967, Volume: 1

Functional dissection of the human spumaretrovirus transactivator identifies distinct classes of dominant-negative mutants.

Content Provider	PubMed Central
Author	Venkatesh, L. K. Yang, C. Theodorakis, P. A. Chinnadurai, G.
Abstract	The bel1 gene of human spumaretrovirus (HSRV) codes for a 300-amino-acid nuclear protein, termed Bel1, that can strongly activate transcription from the cognate long terminal repeat (LTR) by at least 200-fold. Bel1 can also activate human immunodeficiency virus type 1 (HIV-1) LTR expression. By using site-directed mutagenesis, we have identified distinct regions of Bel1 essential for HSRV LTR activation. The amino-terminal 55 residues, which comprise a highly acidic region followed by a short basic stretch, were dispensable for activation. The distribution of functionally defective mutants indicates that two distinct regions between residues 56 and 300 cooperate to confer full activator function. The larger, more amino-terminal region between residues 56 and 227 is sufficient to minimally activate the HSRV LTR. It contains a region between residues 88 and 110 that is strongly conserved between the simian and human spumavirus transactivators but otherwise lacks obvious homology to known transcriptional activators except for an Arg-rich nuclear localization sequence (NLS) between residues 211 and 225 that can be functionally substituted for by the NLS of the simian virus 40 large T antigen. The carboxy-terminal 73 residues contain two functionally redundant regions that can independently augment the activity of the more N-terminal minimal activator domain by 30- to 90-fold. Comparative analysis of the effect of Bel1 mutations on HSRV and HIV-1 LTR expression revealed a similar requirement of Bel1 domains for activation of the two LTRs. Bel1 is phosphorylated in vivo, and a nuclear localization-defective mutant lacking residues 211 to 222 was severely defective for phosphorylation, whereas various deletion mutations in residues 228 to 300 resulted in a four- to eightfold reduction in phosphate incorporation. When functionally defective bel1 mutants were examined for a dominant-negative phenotype, only mutants lacking a proline-rich basic region between residues 194 and 200 or the NLS between residues 211 and 222 that were found to occupy predominantly nuclear and cytoplasmic locations, respectively, could suppress wild-type Bel1 function efficiently. In identifying two classes of dominant-negative mutants with distinct subcellular localization phenotypes, the mutational analysis of Bel1 has revealed a feature unusual for known transcriptional activators.
Starting Page	161
File Format	PDF
ISSN	10985514
e-ISSN	10985514
Journal	Journal of Virology
Issue Number	1
Volume Number	67
Language	English
Publisher Date	1993-01-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology Insect Science

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in