NDLI: Circumvention of ara-C resistance by aphidicolin in blast cells from patients with AML

Content Provider	PubMed Central
Author	Sargent, J. M. Elgie, A. W. Williamson, C. J. Lewandowicz, G. M. Taylor, C. G.
Copyright Year	2001
Abstract	Treatment failure in AML is often attributed to P-glycoprotein-associated multidrug resistance. However, the importance of increased DNA repair in resistant cells is becoming more apparent. In order to investigate the ability of the DNA repair inhibitor aphidicolin to modulate drug resistance, we continually exposed blasts cells, isolated from 22 patients with AML, to a variety of agents ± 15 μM aphidicolin for 48 hours. Cell survival was measured using the MTT assay. Overall, there was no significant effect of aphidicolin on sensitivity to daunorubicin, doxorubicin, etoposide or fludarabine. However, there was a marked increase in sensitivity to ara-C with a median 4.75-fold increase overall (range 0.8–80-fold;P< 0.005). The effect of aphidicolin was significantly greater in blast cells found resistant in vitro to ara-C (8.9-fold compared to 2.12-fold, P< 0.01). This observation was further validated by the correlation between ara-C LC 50 and extent of modulation effect (P< 0.05). Cells isolated from 10 cord blood samples were also tested in order to establish the haematological toxicity of combining ara-C and aphidicolin. The therapeutic index (LC 50 normal cells/tumour cells) for ara-C + aphidicolin was higher than that for ara-C alone suggesting no increased myelotoxicity for the combination. Increased cytotoxicity without increased haematotoxicity makes the combination of ara-C plus aphidicolin ideal for inclusion in future clinical trials. © 2001 Cancer Research Campaign http://www.bjcancer.com
Related Links	http://dx.doi.org/10.1054/bjoc.2000.1639
Ending Page	685
Page Count	6
Starting Page	680
File Format	PDF
ISSN	15321827
e-ISSN	15321827
Journal	British Journal of Cancer
Issue Number	5
Volume Number	84
Language	English
Publisher	Nature Publishing Group
Publisher Date	2001-03-01
Access Restriction	Open
Rights Holder	Nature Publishing Group
Subject Keyword	Cancer Research Oncology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology

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