NDLI: Natural antibody-induced intracellular signalling and growth control in C3H 10T½ fibroblast variants

Content Provider	PubMed Central
Author	Wang, H. Chow, D. A.
Copyright Year	2000
Abstract	Cell-surface binding by natural antibody (NAb) places it well for controlling cell function directly through signalling. Flow cytometry revealed an instability of syngeneic NAb binding to C3H 10T½ fibroblast variants at 37°, which could be partially reduced by H7, an inhibitor of the pivotal signalling serine/threonine kinase, protein kinase C (PKC). Cells coated with purified NAb at 4° followed by a rise in temperature to 37° showed an increase in membrane expression of introduced rat PKC-β1 and endogenous PKC-α, in the PKC-β1-overexpressing PKC-4 and v-H-ras-producing I3T2.1, respectively. Tyrosine phosphorylation of membrane-associated 60 000 MW protein including the tyrosine kinase src was markedly reduced. In addition, both the precoated NAb and numerous membrane molecules ranging from 20 000 to 220 000 MW were released into the supernatant, including the receptor-like protein tyrosine phosphatase α (RPTP-α). Furthermore, purified NAb reduced the growth of I3T2.1 cells in culture assessed as a decrease in total cell numbers and an increase in the proportion of cells in the G0/G1 phase of the cell cycle. Together, these data argue that the interaction of NAb with cell surface structures initiated a series of intracellular signalling events leading to the release of membrane molecules and over time the suppression of cell proliferation. This process could provide a biological mechanism for direct NAb control of activated cells in both physiological and pathological conditions.
Related Links	http://dx.doi.org/10.1046/j.1365-2567.2000.00131.x
Ending Page	466
Page Count	9
Starting Page	458
File Format	PDF
ISSN	00192805
e-ISSN	13652567
Journal	Immunology
Issue Number	4
Volume Number	101
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2000-12-01
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Immunology Immunology and Allergy Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Fibroblast growth factor receptor 1 is principally responsible for fibroblast growth factor 2-induced catabolic activities in human articular chondrocytes

Immunoregulation of lung fibroblast growth: alteration in asbestos-induced pulmonary fibrosis.

Species-specific engagement of human nucleotide oligomerization domain 2 (NOD)2 and Toll-like receptor (TLR) signalling upon intracellular bacterial infection: role of Crohn’s associated NOD2 gene variants

Antibody Production, Anaphylactic Signs, and T-Cell Responses Induced by Oral Sensitization With Ovalbumin in BALB/c and C3H/HeOuJ Mice

Characterization of antibody variants during process development

Intracellular calcium signalling patterns reflect the differentiation status of human T cells

Session 15: Intracellular signalling and development of immune cells (BSI Developmental Immunology Group)

Fibroblast Growth Factor Homologous Factors Are Intracellular Signalling Proteins

Rhinovirus-induced basic fibroblast growth factor release mediates airway remodeling features

Natural antibody-induced intracellular signalling and growth control in C3H 10T½ fibroblast variants

Similar Documents

Fibroblast growth factor receptor 1 is principally responsible for fibroblast growth factor 2-induced catabolic activities in human articular chondrocytes

Immunoregulation of lung fibroblast growth: alteration in asbestos-induced pulmonary fibrosis.

Species-specific engagement of human nucleotide oligomerization domain 2 (NOD)2 and Toll-like receptor (TLR) signalling upon intracellular bacterial infection: role of Crohn’s associated NOD2 gene variants

Antibody Production, Anaphylactic Signs, and T-Cell Responses Induced by Oral Sensitization With Ovalbumin in BALB/c and C3H/HeOuJ Mice

Characterization of antibody variants during process development

Intracellular calcium signalling patterns reflect the differentiation status of human T cells

Session 15: Intracellular signalling and development of immune cells (BSI Developmental Immunology Group)

Fibroblast Growth Factor Homologous Factors Are Intracellular Signalling Proteins

Rhinovirus-induced basic fibroblast growth factor release mediates airway remodeling features

Natural antibody-induced intracellular signalling and growth control in C3H 10T½ fibroblast variants