NDLI: Induction of cell proliferation in quiescent NIH 3T3 cells by oncogenic c-Raf-1.

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Induction of cell proliferation in quiescent NIH 3T3 cells by oncogenic c-Raf-1.

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Frameshift intermediates in homopolymer runs are removed efficiently by yeast mismatch repair proteins.

Microsatellite instability in yeast: dependence on repeat unit size and DNA mismatch repair genes.

Hypermutability of homonucleotide runs in mismatch repair and DNA polymerase proofreading yeast mutants.

The repeat organizer, a specialized insulator element within the intergenic spacer of the Xenopus rRNA genes.

DNA binding by MADS-box transcription factors: a molecular mechanism for differential DNA bending.

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Evolutionary conservation of regulatory strategies for the sex determination factor transformer-2.

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Induction of cell proliferation in quiescent NIH 3T3 cells by oncogenic c-Raf-1.

Content Provider	PubMed Central
Author	Kerkhoff, E. Rapp, U. R.
Abstract	The c-Raf-1 kinase is activated by different mitogenic stimuli and has been shown to be an important mediator of growth factor responses. Fusion of the catalytic domain of the c-Raf-1 kinase with the hormone binding domain of the estrogen receptor (deltaRaf-ER) provides a hormone-regulated form of oncogenic activated c-Raf-1. We have established NIH 3T3 cells stably expressing a c-Raf-1 deletion mutant-estrogen receptor fusion protein (c-Raf-1-BxB-ER) (N-BxB-ER cells). The transformed morphology of these cells is dependent on the presence of the estrogen antagonist 4-hydroxytamoxifen. Addition of 4-hydroxytamoxifen to N-BxB-ER cells arrested by density or serum starvation causes reentry of these cells into cell proliferation. Increases in the cell number are obvious by 24 h after activation of the oncogenic c-Raf-1 protein in confluent cells. The onset of proliferation in serum-starved cells is further delayed and takes about 48 h. In both cases, the proliferative response of the oncogenic c-Raf-1-induced cell proliferation is weaker than the one mediated by serum and does not lead to exponential growth. This is reflected in a markedly lower expression of the late-S- and G2/M-phase-specific cyclin B protein and a slightly lower expression of the cyclin A protein being induced at the G1/S transition. Oncogenic activation of c-Raf-1 induces the expression of the heparin binding epidermal growth factor. The Jnk1 kinase is putatively activated by the action of the autocrine growth factor. The kinetics of Jnk1 kinase activity is delayed and occurs by a time when we also detect DNA synthesis and the expression of the S-phase-specific cyclin A protein. This finding indicates that oncogenic activation of the c-Raf-1 protein can trigger the entry into the cell cycle without the action of the autocrine growth factor loop. The activation of the c-Raf-1-BxB-ER protein leads to an accumulation of high levels of cyclin D1 protein and a repression of the p27Kip1 cyclin-dependent kinase inhibitor under all culture conditions tested.
Starting Page	2576
File Format	PDF
ISSN	10985549
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	5
Volume Number	17
Language	English
Publisher Date	1997-05-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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