NDLI: Optimal combination of media for primary isolation of Helicobacter pylori from gastric biopsy specimens.

Please wait, while we are loading the content...

Year: 2016, Volume: 54

Year: 2015, Volume: 53

Year: 2014, Volume: 52

Year: 2013, Volume: 51

Year: 2012, Volume: 50

Year: 2011, Volume: 49

Year: 2010, Volume: 48

Year: 2009, Volume: 47

Year: 2008, Volume: 46

Year: 2007, Volume: 45

Year: 2006, Volume: 44

Year: 2005, Volume: 43

Year: 2004, Volume: 42

Year: 2003, Volume: 41

Year: 2002, Volume: 40

Year: 2001, Volume: 39

Year: 2000, Volume: 38

Year: 1999, Volume: 37

Year: 1998, Volume: 36

Year: 1997, Volume: 35

Year: 1997, Volume: 35, Issue: 12

Year: 1997, Volume: 35, Issue: 11

Year: 1997, Volume: 35, Issue: 10

Year: 1997, Volume: 35, Issue: 9

Year: 1997, Volume: 35, Issue: 8

Year: 1997, Volume: 35, Issue: 7

Year: 1997, Volume: 35, Issue: 6

Direct detection and identification of Pseudomonas aeruginosa in clinical samples such as skin biopsy specimens and expectorations by multiplex PCR based on two outer membrane lipoprotein genes, oprI and oprL.

Vector potential of houseflies (Musca domestica) for Helicobacter pylori.

PCR detection of human papillomavirus: comparison between MY09/MY11 and GP5+/GP6+ primer systems.

Variability in susceptibilities of Haemophilus influenzae to clarithromycin and azithromycin due to medium pH.

Molecular epidemiology of antibiotic resistance of Salmonella enteritidis during a 7-year period in Greece.

PCR and probe-PCR assays to monitor broodstock Atlantic salmon (Salmo salar L.) ovarian fluid and kidney tissue for presence of DNA of the fish pathogen Renibacterium salmoninarum.

Prevalence of Bartonella henselae and Bartonella clarridgeiae in stray cats.

Comparative analysis of genetic variability among Candida albicans isolates from different geographic locales by three genotypic methods.

Rapid identification and fingerprinting of Candida krusei by PCR-based amplification of the species-specific repetitive polymorphic sequence CKRS-1.

Equally high prevalences of infection with cagA-positive Helicobacter pylori in Chinese patients with peptic ulcer disease and those with chronic gastritis-associated dyspepsia.

Detection of Treponema pallidum by a sensitive reverse transcriptase PCR.

Detection and identification of fungal pathogens in blood by using molecular probes.

Fatty acid characterization of rapidly growing pathogenic aerobic actinomycetes as a means of identification.

Detection of Chlamydia trachomatis in male and female urine specimens by using the amplified Chlamydia trachomatis test.

A new PCR-based seroneutralization assay in cell culture for diagnosis of hepatitis E.

Characterization of Bacteroides forsythus isolates.

Comparison of BACTEC 9240 Peds Plus medium and isolator 1.5 microbial tube for detection of Brucella melitensis from blood cultures.

Comparison of two automated DNA amplification systems with a manual one-tube nested PCR assay for diagnosis of pulmonary tuberculosis.

Genetic characterization of multidrug-resistant Mycobacterium bovis strains from a hospital outbreak involving human immunodeficiency virus-positive patients.

Survival of Acinetobacter baumannii on dry surfaces.

Comparison of biotyping, ribotyping, and pulsed-field gel electrophoresis for investigation of a common-source outbreak of Burkholderia pickettii bacteremia.

Analysis of incidence of infection with enterotoxigenic Escherichia coli in a prospective cohort study of infant diarrhea in Nicaragua.

Determination of genotypes of Toxoplasma gondii strains isolated from patients with toxoplasmosis.

Contribution of a typing method based on IS256 probing of SmaI-digested cellular DNA to discrimination of European phage type 77 methicillin-resistant Staphylococcus aureus strains.

A simple method for determining metronidazole resistance of Helicobacter pylori.

Etiological agents of infectious diarrhea: implications for requests for microbial culture.

Interpretation criteria for standardized Western blots for three European species of Borrelia burgdorferi sensu lato.

Usefulness of PCR for diagnosis of Pneumocystis carinii pneumonia in different patient groups.

Diagnosis of toxoplasmosis by joint detection of immunoglobulin A and immunoglobulin M.

Rapid identification of Candida species in blood cultures by a clinically useful PCR method.

Rapid detection of oxacillin-resistant Staphylococcus aureus in blood cultures by an impedance method.

Prevalence of granulocytic Ehrlichia infection among white-tailed deer in Wisconsin.

Clinical comparison of BACTEC 9240 plus aerobic/F resin bottles and the isolator aerobic culture system for detection of bloodstream infections.

Effects of incubation time and buffer concentration on in vitro activities of antifungal agents against Candida albicans.

Detection of Mycobacterium tuberculosis directly from sputum by using a prototype automated Q-beta replicase assay.

Performance of an automated Q-beta replicase amplification assay for Mycobacterium tuberculosis in a clinical trial.

Multiplex PCR assay for immediate identification of the infecting species in patients with mycobacterial disease.

Relationship between adhesion to intestinal Caco-2 cells and multidrug resistance in Klebsiella pneumoniae clinical isolates.

Detection and typing of herpes simplex viruses by using recombinant immunoglobulin fragments produced in bacteria.

An indirect immunofluorescence assay using a cell culture-derived antigen for detection of antibodies to the agent of human granulocytic ehrlichiosis.

Outbreak of amoxicillin-resistant Haemophilus influenzae type b: variable number of tandem repeats as novel molecular markers.

Random amplification of polymorphic DNA reveals serotype-specific clonal clusters among enterotoxigenic Escherichia coli strains isolated from humans.

Evaluation of nine immunoassay kits (enzyme immunoassay and direct fluorescence) for detection of Giardia lamblia and Cryptosporidium parvum in human fecal specimens.

Evaluation of a rapid enzyme immunoassay for diagnosis of hepatic amoebiasis.

Use of pulsed-field gel electrophoresis to investigate a pseudo-outbreak of Bacillus cereus in a pediatric unit.

Detection of Neisseria gonorrhoeae and Chlamydia trachomatis in genitourinary specimens from men and women by a coamplification PCR assay.

Optimal combination of media for primary isolation of Helicobacter pylori from gastric biopsy specimens.

Oligonucleotide (GTG)5 as an epidemiological tool in the study of nontuberculous mycobacteria.

Molecular characterization of two Bordetella bronchiseptica strains isolated from children with coughs.

Human disease in Europe caused by a granulocytic Ehrlichia species.

Detection of human papillomavirus type 16 early-gene transcription by reverse transcription-PCR is associated with abnormal cervical cytology.

Emergence and dissemination of a highly vancomycin-resistant vanA strain of Enterococcus faecium at a large teaching hospital.

Detection of hydatid antigen in urine by countercurrent immunoelectrophoresis.

Dot immunoperoxidase assay for detection of parvovirus B19 antigens in serum samples.

Cost-effectiveness of rapid diagnosis of viral respiratory tract infections in pediatric patients.

Investigation of a pseudo-outbreak of Nocardia asteroides infection by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA PCR.

Rapid discrimination of Mycobacterium avium strains from AIDS patients by randomly amplified polymorphic DNA analysis.

Detection of Pneumocystis carinii in respiratory specimens by PCR-solution hybridization enzyme-linked immunoassay.

Rapid detection of Mycoplasma pneumoniae by an assay based on PCR and probe hybridization in a nonradioactive microwell plate format.

Demonstration of vertical transmission of Streptococcus suis in swine by genomic fingerprinting.

A single-tube nested PCR for Pneumocystis carinii f. sp. hominis.

Detection of human Betaherpesvirinae in saliva and urine from immunocompromised and immunocompetent subjects.

Evaluation of the COBAS AMPLICOR MTB system.

Serological diagnosis of bovine, caprine, and ovine mastitis caused by Listeria monocytogenes by using an enzyme-linked immunosorbent assay.

Use of heteroduplex analysis to classify legionellae on the basis of 5S rRNA gene sequences.

Use of quantitative competitive PCR to measure Epstein-Barr virus genome load in the peripheral blood of pediatric transplant patients with lymphoproliferative disorders.

Endocarditis caused by nonhemolytic group B streptococcus.

Validity of self-obtained vaginal specimens for diagnosis of trichomoniasis.

Fecal PCR assay for diagnosis of Helicobacter infection in laboratory rodents.

A novel method for isolation of Chlamydia pneumoniae by treatment with trypsin or EDTA.

Comparison of radioimmunoassay and enzyme immunoassay kits for detection of Legionella pneumophila serogroup 1 antigen in both concentrated and nonconcentrated urine samples.

Rapid identification of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, and Haemophilus paraphrophilus by restriction enzyme analysis of PCR-amplified 16S rRNA genes.

Rapid detection of Vibrio cholerae O139 Bengal from stool specimens by PCR.

Development of a highly specific assay for rapid identification of pathogenic strains of Yersinia enterocolitica based on PCR amplification of the Yersinia heat-stable enterotoxin gene (yst).

Coli surface antigens associated with enterotoxigenic Escherichia coli strains isolated from persons with traveler's diarrhea in Asia.

Prevalence of the virulence-associated gene of Rhodococcus equi in isolates from infected foals.

Coinfection with Tropheryma whippelii and a Whipple's disease-associated bacterial organism detected in a patient with central nervous system Whipple's disease.

Amplification and restriction endonuclease digestion of a large fragment of genes coding for rRNA as a rapid method for discrimination of closely related pathogenic bacteria.

Year: 1997, Volume: 35, Issue: 5

Year: 1997, Volume: 35, Issue: 4

Year: 1997, Volume: 35, Issue: 3

Year: 1997, Volume: 35, Issue: 2

Year: 1997, Volume: 35, Issue: 1

Year: 1996, Volume: 34

Year: 1995, Volume: 33

Year: 1994, Volume: 32

Year: 1993, Volume: 31

Year: 1992, Volume: 30

Year: 1991, Volume: 29

Year: 1990, Volume: 28

Year: 1989, Volume: 27

Year: 1988, Volume: 26

Year: 1987, Volume: 25

Year: 1986, Volume: 24

Year: 1986, Volume: 23

Year: 1985, Volume: 22

Year: 1985, Volume: 21

Year: 1984, Volume: 20

Year: 1984, Volume: 19

Year: 1983, Volume: 18

Year: 1983, Volume: 17

Year: 1982, Volume: 16

Year: 1982, Volume: 15

Year: 1981, Volume: 14

Year: 1981, Volume: 13

Year: 1980, Volume: 12

Year: 1980, Volume: 11

Year: 1979, Volume: 10

Year: 1979, Volume: 9

Year: 1978, Volume: 8

Year: 1978, Volume: 7

Year: 1977, Volume: 6

Year: 1977, Volume: 5

Year: 1976, Volume: 4

Year: 1976, Volume: 3

Year: 1975, Volume: 2

Year: 1975, Volume: 1

Optimal combination of media for primary isolation of Helicobacter pylori from gastric biopsy specimens.

Content Provider	PubMed Central
Author	Piccolomini, R. Bonaventura, G. Di Festi, D. Catamo, G. Laterza, F. Neri, M.
Abstract	The aim of the present study was to compare eight media, four nonselective and four selective media, to determine the best combination of media for the primary isolation of Helicobacter pylori. Over a period of 5 months, mucosal antral biopsy specimens were obtained from 222 consecutive dyspeptic patients undergoing endoscopy. Biopsy samples were plated in parallel on all eight media. Egg yolk emulsion agar (EYE), Skirrow's medium, Dent's medium, and modified Thayer-Martin medium were used as selective media; modified chocolate agar (MCHOC), Triptycase soy agar (TSA), brucella agar, and brain heart infusion agar were used as nonselective media. Overall, by using these eight media, H. pylori was recovered from biopsy specimens from 114 of 222 patients, yielding an isolation rate of 51%. Comparison of all possible combinations of the eight media showed that the highest rate of isolation of H. pylori was 100% (114 of 114) with EYE-MCHOC, followed by 96.5% (110 of 114) when EYE-TSA was used. Conversely, it was found that none of the media used alone yielded a 100% rate of recovery (the maximum recovery rate was 95%, which was achieved with EYE). These results indicate that the association of EYE and MCHOC yielded the maximum recovery of H. pylori from gastric biopsy specimens. Therefore, the use of selective and nonselective media in parallel offers optimal recovery rates with only a slight increase in costs.
Starting Page	1541
File Format	PDF
ISSN	1098660X
e-ISSN	1098660X
Journal	Journal of Clinical Microbiology
Issue Number	6
Volume Number	35
Language	English
Publisher Date	1997-06-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Microbiology (medical)

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in