NDLI: Catecholamines are not linked to myometrial phospholipase C and uterine contraction in late pregnant and parturient mouse

Content Provider	PubMed Central
Author	Mhaouty-kodja, Sakina Eric, Houdeau Cohen Tannoudji, Joëlle Legrand, Chantal
Copyright Year	2001
Abstract	We investigated whether catecholamines through activation of α1-adrenergic receptors (α1-AR) are involved in mouse uterine contraction at parturition. Myometrial phospholipase C (PLC) activity and uterine contraction were measured in response to noradrenaline (NA), the specific α1-AR agonist phenylephrine (Phe) and oxytocin (OT). Using the reverse transcription-polymerase chain reaction RT-PCR, we detected the α1a-AR subtype in late pregnant mouse myometrium. We also detected, by immunoblotting studies, PLCβ1, PLCβ3 and different α-subunits of pertussis toxin-insensitive (Gαq/11) and -sensitive G proteins (Gαo/i3, Gαi1/2). Phenylephrine and NA did not alter the myometrial inositol phosphate (InsP) production of late pregnant or parturient mouse. In similar conditions, OT increased InsP production in a dose-dependent manner. Consistent with these results, only OT (10 μm) recruited PLCβ1 and PLCβ3 to myometrial plasma membranes. The OT-induced InsP response was not altered by pertussis toxin (300 ng ml−1, 2 h pretreatment), suggesting the involvement of a member of the Gαq family. Noradrenaline and Phe failed to increase uterine contraction at late pregnancy and at parturition. In contrast, OT induced uterine contraction in a dose-dependent manner with maximal increase (400 %) at a concentration of 1 μm. The results indicate that OT receptors (OTR) but not α1-AR are linked to myometrial PLC activation and uterine contraction in late pregnant and parturient mouse. This discrepancy between mouse and other mammals could be attributed to the α1-AR subtype expressed in myometrium at this time.
Related Links	http://dx.doi.org/10.1111/j.1469-7793.2001.00123.x
Ending Page	131
Page Count	9
Starting Page	123
File Format	PDF
ISSN	00223751
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 1
Volume Number	536
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2001-10-01
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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