NDLI: Dominant Suppression of Repeat-Induced Point Mutation in Neurospora crassa by a Variant Catalytic Subunit of DNA Polymerase-ζ

Content Provider	PubMed Central
Author	Ranjan, Tamuli Kasbekar, Durgadas P.
Abstract	Crosses involving the Adiopodoumé strain of Neurospora crassa are defective for repeat-induced point mutation (RIP), a genome defense mechanism of fungi. We show here that the Adiopodoumé strain possesses an incompletely penetrant and variably expressive dominant suppressor of RIP (Srp) that maps to an ∼34-kbp genome segment that is ∼26 kbp proximal to mat on linkage group IL. Gene disruption experiments revealed that Srp is the upr-1 allele of Adiopodoumé (upr-1Ad ) that is contained within this segment. The upr-1 gene codes for the catalytic subunit of the translesion DNA polymerase-ζ (Pol-ζ) and it is unusually polymorphic in Neurospora. That the upr-1 gene contains upstream ORFs that overlap with the main ORF is potentially relevant to the incomplete penetrance and variable expressivity of the suppressor. Crosses between heterokaryons that contain upr-1Ad and strains that prevent mating events involving nuclei that contain upr-1Ad yielded no progeny in which RIP had occurred, consistent with the idea that the suppressor encoded by upr-1Ad is diffusible. The potential involvement of the Pol-ζ subunit in two functions, translesion DNA synthesis and RIP regulation, might account for the rapid evolution of its gene in Neurospora.
Related Links	http://dx.doi.org/10.1534/genetics.107.079483
Ending Page	1176
Page Count	8
Starting Page	1169
File Format	PDF
ISSN	00166731
Journal	Genetics
Issue Number	3
Volume Number	178
Language	English
Publisher	Genetics Society of America
Publisher Date	2008-02-01
Access Restriction	Open
Rights Holder	Genetics Society of America
Subject Keyword	Genetics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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