NDLI: Oxygen-sensing persistent sodium channels in rat hippocampus

Content Provider	PubMed Central
Author	Hammarström, Anna K. M. Gage, Peter W.
Copyright Year	2000
Abstract	Persistent sodium channel activity was recorded before and during hypoxia from cell-attached and inside-out patches obtained from cultured hippocampal neurons at a pipette potential (V p) of +30 mV. Average mean current (I′) of these channels was very low under normoxic conditions and was similar in cell-attached and excised inside-out patches (-0.018 ± 0.010 and -0.025 ± 0.008 pA, respectively, n = 24). Hypoxia increased the activity of persistent sodium channels in 10 cell-attached patches (I’ increased from -0.026 ± 0.016 pA in control to -0.156 ± 0.034 pA during hypoxia, n = 4, P = 0.013). The increased persistent sodium channel activity was most prominent at a V P between +70 and +30 mV (membrane potential, V m = -70 to -30 mV) and could be blocked by lidocaine, TTX or R56865 (n = 5). Sodium cyanide (NaCN, 5 mM; 0.5-5 min) increased persistent sodium channel activity in cell-attached patches (n = 3) in a similar manner. Hypoxia also increased sodium channel activity in inside-out patches from hippocampal neurons. Within 2-4 min of exposure to hypoxia, I′ had increased 9-fold to -0.18 ± 0.04 pA (n = 21, P = 0.001). Sodium channel activity increased further with longer exposures to hypoxia. The hypoxia-induced sodium channel activity in inside-out patches could be inhibited by exposure to 10-100 μM lidocaine applied via the bath solution (I′ = -0.03 ± 0.01 pA, n = 8) or by perfusion of the pipette tip with 1 μM TTX (I′ = -0.01 ± 0.01 pA, n = 3). The reducing agent dithiothreitol (DTT, 2-5 mM) rapidly abolished the increase in sodium channel activity caused by hypoxia in excised patches (I’ = -0.01 ± 0.01 pA, n = 4). Similarly, reduced glutathione (GSH, 5-20 mM) also reversed the hypoxia-induced increase in sodium channel activity (I’ = -0.02 ± 0.02 pA, n = 5). These results suggest that persistent sodium channels in neurons can sense O2 levels in excised patches of plasma membrane. Hypoxia triggers an increase in sodium channel activity. The redox reaction involved in increasing the sodium channel activity probably occurs in an auxiliary regulatory protein, co-localized in the plasma membrane.
Related Links	http://dx.doi.org/10.1111/j.1469-7793.2000.00107.x
Ending Page	118
Page Count	12
Starting Page	107
File Format	PDF
ISSN	00223751
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 1
Volume Number	529
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2000-11-01
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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