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| Content Provider | PubMed Central |
|---|---|
| Author | Sokolov, Stanislav Weiß, Regina G. Timin, Evgeni N. Hering, Steffen |
| Copyright Year | 2000 |
| Abstract | β-subunit modulation of slow inactivation of class A calcium (Ca2+) channels was studied with two-microlectrode voltage clamp after expression of the α1A- (BI-2) together with β1a-, β2a-, β3- or β4-subunits in Xenopus oocytes. On- and off-rates of slow inactivation were estimated from the kinetics of recovery from slow inactivation. Ca2+ channels with an α1A/β-subunit composition inducing the slower rate of fast inactivation displayed the faster rate of slow inactivation. The corresponding order of slow inactivation time constants (τonset) was: α1A/β2a, 33 ± 3 s; α1A/β4, 42 ± 4 s; α1A/β1a, 59 ± 4 s; α1A/β3, 67 ± 5 s (n ≥ 7). Recovery of class A Ca2+ channels from slow inactivation was voltage dependent and accelerated at hyperpolarized voltages. At a given holding potential recovery kinetics were not significantly modulated by different β-subunits. Two mutations in segment IIIS6 (IF1612/1613AA) slowed fast inactivation and accelerated the onset of slow inactivation in the resulting mutant (α1A/IF-AA/β3) in a similar manner as coexpression of the β2a-subunit. Recovery from slow inactivation was slightly slowed in the double mutant. Our data suggest that class A Ca2+ channels enter the ‘slow inactivated’ state more willingly from the open than from the ‘fast inactivated’ state. The rate of slow inactivation is, therefore, indirectly modulated by different β-subunits. Fast and slow inactivation in class A Ca2+ channels appears to represent structurally independent conformational changes. Fast inactivation is not a prerequisite for slow inactivation. |
| Related Links | http://dx.doi.org/10.1111/j.1469-7793.2000.t01-1-00445.x |
| Starting Page | 445 |
| File Format | |
| ISSN | 14697793 |
| e-ISSN | 14697793 |
| Journal | The Journal of Physiology |
| Issue Number | Pt 3 |
| Volume Number | 527 |
| Language | English |
| Publisher | Blackwell Science Inc |
| Publisher Date | 2000-09-15 |
| Access Restriction | Open |
| Rights Holder | Blackwell Science Inc |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Sports Science |
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