NDLI: Differences in Ca2+ buffering properties between excitatory and inhibitory hippocampal neurons from the rat

Content Provider	PubMed Central
Author	Lee, Suk-ho Rosenmund, Christian Schwaller, Beat Neher, Erwin
Copyright Year	2000
Abstract	Endogenous calcium binding ratios (κS) in dendrites of cultured hippocampal neurons were estimated according to the single compartment model for transients in intracellular Ca2+ concentration ([Ca2+]). In addition, the electrophysiological characteristics of neurons were classified by their autaptic currents and intrinsic firing patterns. These data were analysed in order to determine whether a correlation between Ca2+ buffers and electrophysiological type exists. Ca2+ binding ratios of endogenous buffers were estimated by eliciting [Ca2+] transients with short depolarizations, while cells were loaded with fura-2. Two types of estimates could be obtained: one termed κS(τ), based on analysing time constants (τ) of [Ca2+] transients, and another termed κS(dCa), derived from an analysis of initial amplitudes of [Ca2+] transients. Values for κS(τ) and κS(dCa) were estimated as 57 ± 10 (mean ±s.d., n = 10) and 60 ± 14 (n = 10), respectively, in excitatory neurons, and 130 ± 50 (n = 11) and 150 ± 70 (n = 11), respectively, in inhibitory neurons. The κS values of excitatory and inhibitory cells were significantly different from each other, regardless of the measurement method (Student's t test, P < 0.01). However, there was no significant difference in κS between the groups classified according to firing patterns. Although κS(τ) values were well matched to those of κS(dCa) in most excitatory cells, the two values did not agree in three out of the fourteen inhibitory cells investigated. In these cells, the first few [Ca2+] transients after obtaining the whole cell configuration displayed a double exponential decay, suggesting that buffers with slow binding kinetics, such as parvalbumin, are involved. This hypothesis is further explored in an accompanying paper.
Related Links	http://dx.doi.org/10.1111/j.1469-7793.2000.t01-3-00405.x
Ending Page	418
Page Count	14
Starting Page	405
File Format	PDF
ISSN	00223751
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 2
Volume Number	525
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2000-06-01
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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