NDLI: In vivo characterization of renal iron transport in the anaesthetized rat

Content Provider	PubMed Central
Author	Wareing, Mark Ferguson, Carole J. Green, Roger Riccardi, Daniela Smith, Craig P.
Copyright Year	2000
Abstract	In vivo microinjections of 55FeCl3 were made to assess renal iron (Fe2+/3+) transport in the anaesthetized rat. Following microinjection into proximal convoluted tubules (PCTs), 18·5 ± 2·9 % (mean ± s.e.m., n = 11) of the 55Fe was recovered in the urine. This recovery was not dependent on the injection site indicating that iron is not reabsorbed across the surface convolutions of the proximal tubule. Following microinjection into distal convoluted tubules (DCTs) 46·1 ± 6·1 % (n = 8) of the injected 55Fe was recovered. Taken together the recovery data from the PCT and DCT microinjection studies indicate that the transport of iron occurs in the loop of Henle (LH) and collecting duct system. In vivo luminal microperfusion was used to examine iron transport by the LH in more detail. In tubules perfused with 7 μmol l−1 55FeCl3, 52·7 ± 8·3 % (n = 8) of the perfused 55Fe was recovered in the collected fluid, indicating significant iron reabsorption in the LH. Addition of copper (Cu2+ as 7 μmol l−1 CuSO4), manganese (Mn2+ as 7 μmol l−1 MnSO4) or zinc (Zn2+ as 7 μmol l−1 ZnSO4) to the perfusate did not affect reabsorption of water, Na+ or K+, but increased recovery of 55Fe to 83·5 ± 6·8 % (n = 8, P < 0·04), 75·8 ± 5·9 (n = 6, not significant, n.s.) and 67·9 ± 3·8; (n = 9, n.s.), respectively. Thus, iron transport in the LH can be reduced by the addition of copper or manganese to the luminal perfusate suggesting that these ions may compete with iron for a common transport pathway. However, this pathway may not be shared by zinc.
Related Links	http://dx.doi.org/10.1111/j.1469-7793.2000.00581.x
Starting Page	581
File Format	PDF
ISSN	14697793
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 2
Volume Number	524
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2000-04-15
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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