NDLI: 13C NMR study of the effects of leptin treatment on kinetics of hepatic intermediary metabolism

Content Provider	PubMed Central
Author	Cohen, Sheila M. Werrmann, Jeffrey G. Tota, Michael R.
Copyright Year	1998
Abstract	The recent discovery of leptin receptors in peripheral tissue raises questions about which of leptin’s biological actions arise from direct effects of the hormone on extraneural tissues and what intracellular mechanisms are responsible for leptin’s effects on carbohydrate and lipid metabolism. The present study is focused on the action of leptin on hepatic metabolism. Nondestructive 13C NMR methodology was used to follow the kinetics of intermediary metabolism by monitoring flux of 13C-labeled substrate through several multistep pathways. In perfused liver from either ob/ob or lean mice, we found that acute treatment with leptin in vitro modulates pathways controlling carbohydrate flux into 13C-labeled glycogen, thereby rapidly enhancing synthesis by an insulin-independent mechanism. Acute treatment of ob/ob liver also caused a rapid stimulation of long-chain fatty acid synthesis from 13C-labeled acetyl-CoA by the de novo synthesis route. Chronic leptin treatment in vivo induced homeostatic changes that resulted in a tripling of the rate of glycogen synthesis via the gluconeogenic pathway from [2-13C]pyruvate in ob/ob mouse liver perfused in the absence of the hormone. Consistent with the 13C NMR results, leptin treatment of the ob/ob mouse in vivo resulted in significantly increased hepatic glycogen synthase activity. Chronic treatment with leptin in vivo exerted the opposite effect of acute treatment in vitro and markedly decreased hepatic de novo synthesis of fatty acids in ob/ob mouse liver. In agreement with the 13C NMR findings, activities of hepatic acetyl-CoA carboxylase and fatty acid synthase were significantly reduced by chronic treatment of the ob/ob mouse with leptin. Our data represent a demonstration of direct effects of leptin in the regulation of metabolism in the intact functioning liver.
Starting Page	7385
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	13
Volume Number	95
Language	English
Publisher	The National Academy of Sciences
Publisher Date	1998-06-23
Access Restriction	Open
Rights Holder	The National Academy of Sciences
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

13C NMR study of the effects of leptin treatment on kinetics of hepatic intermediary metabolism.

Effects of a glucokinase activator on hepatic intermediary metabolism: study with 13C-isotopomer-based metabolomics

Effects of ethanol on alanine metabolism in perfused mouse liver studied by 13C NMR.

Effects of hormone and glucose administration on hepatic glucose and glycogen metabolism in vivo. A 13C NMR study.

High-resolution 13C NMR study of pressure effects on the main phase transition in L-alpha-dipalmitoyl phosphatidylcholine vesicles.

Gluconeogenesis in the cow. The effects of a glucocorticoid on hepatic intermediary metabolism

Cryopreservation Effects on Intermediary Metabolism in a Pancreatic Substitute: A 13C Nuclear Magnetic Resonance Study

13C NMR studies of carbon metabolism in the hyphal fungus Aspergillus nidulans.

Kinetics Study of Glucose Acetylation in Starch Polymers by Quantitative 13C NMR spectroscopy.

13C NMR study of the effects of leptin treatment on kinetics of hepatic intermediary metabolism

Similar Documents

13C NMR study of the effects of leptin treatment on kinetics of hepatic intermediary metabolism.

Effects of a glucokinase activator on hepatic intermediary metabolism: study with 13C-isotopomer-based metabolomics

Effects of ethanol on alanine metabolism in perfused mouse liver studied by 13C NMR.

Effects of hormone and glucose administration on hepatic glucose and glycogen metabolism in vivo. A 13C NMR study.

High-resolution 13C NMR study of pressure effects on the main phase transition in L-alpha-dipalmitoyl phosphatidylcholine vesicles.

Gluconeogenesis in the cow. The effects of a glucocorticoid on hepatic intermediary metabolism

Cryopreservation Effects on Intermediary Metabolism in a Pancreatic Substitute: A 13C Nuclear Magnetic Resonance Study

13C NMR studies of carbon metabolism in the hyphal fungus Aspergillus nidulans.

Kinetics Study of Glucose Acetylation in Starch Polymers by Quantitative 13C NMR spectroscopy.

13C NMR study of the effects of leptin treatment on kinetics of hepatic intermediary metabolism