NDLI: Reexamination of the mechanism of hydroxyl radical adducts formed from the reaction between familial amyotrophic lateral sclerosis-associated Cu,Zn superoxide dismutase mutants and H2O2

Content Provider	PubMed Central
Author	Singh, Ravinder J. Karoui, Hakim Gunther, Michael R. Beckman, Joseph S. Mason, Ronald P. Kalyanaraman, B.
Copyright Year	1998
Abstract	Amyotrophic lateral sclerosis (ALS) involves the progressive degeneration of motor neurons in the spinal cord and motor cortex. Mutations to Cu,Zn superoxide dismutase (SOD) linked with familial ALS are reported to increase hydroxyl radical adduct formation from hydrogen peroxide as measured by spin trapping with 5,5′-dimethyl-1-pyrrolline N-oxide (DMPO). In the present study, we have used oxygen-17-enriched water and H2O2 to reinvestigate the mechanism of DMPO/⋅OH formation from the SOD and SOD mutants. The relative ratios of DMPO/⋅ 17OH and DMPO/⋅ 16OH formed in the Fenton reaction were 90% and 10%, respectively, reflecting the ratios of H2 17O2 to H2 16O2. The reaction of the WT SOD with H2 17O2 in bicarbonate/CO2 buffer yielded 63% DMPO/⋅ 17OH and 37% DMPO/⋅ 16OH. Similar results were obtained from the reaction between familial ALS SOD mutants and H2 17O2: DMPO/⋅ 17OH (64%); DMPO/⋅ 16OH (36%) from A4V and DMPO/⋅ 17OH (62%); and DMPO/⋅ 16OH (38%) from G93A. These results were confirmed further by using 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide spin trap, a phosphorylated analog of DMPO. Contrary to earlier reports, the present results indicate that a significant fraction of DMPO/⋅OH formed during the reaction of SOD and familial ALS SOD mutants with H2O2 is derived from the incorporation of oxygen from water due to oxidation of DMPO to DMPO/⋅OH presumably via DMPO radical cation. No differences were detected between WT and mutant SODs, neither in the concentration of DMPO/⋅OH or DEPMPO/⋅OH formed nor in the relative incorporation of oxygen from H2O2 or water.
Starting Page	6675
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	12
Volume Number	95
Language	English
Publisher	The National Academy of Sciences
Publisher Date	1998-06-09
Access Restriction	Open
Rights Holder	The National Academy of Sciences
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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