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| Content Provider | PubMed Central |
|---|---|
| Author | Kim, Sung Joon Koh, Eun-mi Kang, Tong Mook Kim, Young Chul So, Insuk Isenberg, Gerrit Kim, Ki Whan |
| Copyright Year | 1998 |
| Abstract | Ca2+ microfluorometry (100 μm K5 fura-2) and the voltage-clamp technique were combined to study the effect of carbachol (CCh, 50 μm) in inducing currents (I CCh) through non-selective cation channels (NSCCCh) and increments in global cytosolic Ca2+ concentration (Δ[Ca2+]c). In Na+-containing bath solution, I CCh fell from an initial phasic to a subsequent small (5 %) tonic component; Δ[Ca2+]c fell to zero. Tonic I CCh and [Ca2+]c became prominent after substitution of extracellular 140 mm Na+ by 140 mm Cs+. Tonic I CCh and Δ[Ca2+]c were insensitive to intracellular heparin (3 mg ml−1) and ryanodine (4 μm), i.e. they did not depend on Ca2+ release from sarcoplasmic reticulum (SR). Single channel currents of NSCCCh could be resolved in whole-cell recordings. Substitution of Na+ by Cs+ increased NSCCCh activity by one order of magnitude and slope conductance from 22 to 30 pS. Extracellular quinidine (3 μm) reversibly blocked the NSCCCh activity. Both tonic I CCh and tonic Δ[Ca2+]c (a) followed a similar time course of activation, desensitization and facilitation, (b) were reversibly blocked by 3 μm quinidine, and (c) persisted upon block of SR Ca2+ release. A Ca2+ fractional current of tonic I CCh (f Ca) of 0.009 was calculated by comparing the ratio Δ[Ca2+]c (corrected for simultaneous Ca2+ redistribution) over I CCh with depolarization-induced *Δ[Ca2+]c (Δ[Ca2+]c calculated from I Ca induced by a 400 ms depolarization from −60 to 0 mV at 2 mm[Ca2+]o, 145 mm[Cs+]o) over I Ca. f Ca was 0.023 at [Ca2+]o = 4 mm. With 110 mm extracellular CaCl2 and 145 mm intracellular CsCl, I CCh reversed at +19.5 mV suggesting a permeability ratio P Ca/P Cs of 2.8. We conclude that Ca2+ influx through NSCCCh under physiological [Ca2+]o could induce Δ[Ca2+]c. The f Ca was, however, much smaller than the one calculated from the reversal potential. |
| Related Links | http://dx.doi.org/10.1111/j.1469-7793.1998.749ba.x |
| Ending Page | 760 |
| Page Count | 12 |
| Starting Page | 749 |
| File Format | |
| ISSN | 00223751 |
| e-ISSN | 14697793 |
| Journal | The Journal of Physiology |
| Issue Number | Pt 3 |
| Volume Number | 513 |
| Language | English |
| Publisher | Blackwell Science Inc |
| Publisher Date | 1998-12-01 |
| Access Restriction | Open |
| Rights Holder | Blackwell Science Inc |
| Subject Keyword | Physiology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Sports Science |
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