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| Content Provider | PubMed Central |
|---|---|
| Author | Bolton, T. B. Cannell, M. B. Gordienko, D. V. |
| Copyright Year | 1998 |
| Abstract | Spontaneous, localized transient increases in [Ca2+]i (‘Ca2+ sparks’) were observed in about 40 % of fluo-3-loaded myocytes examined using laser scanning confocal microscopy. Ca2+ sparks persisted after application of Cd2+ (200 μm), but were abolished by ryanodine (30 μm) or thapsigargin (0.1 μm), suggesting that they arise from the spontaneous activation of ryanodine receptors (RyR) in the sarcoplasmic reticulum (SR). Ca2+ sparks occurred much more frequently at certain sites (or ‘frequent discharge sites’, FDSs) within any confocal plane of the cell and line-scan imaging revealed a wide variation in their spatial size, amplitude and time course. Some spontaneous local transients were very similar to ‘Ca2+ sparks’ observed in heart, i.e. lasting ∼200 ms with a peak fluorescence ratio of 1.75 ± 0.23 (mean ± s.d., n = 33). Other events were faster and smaller, lasting only ∼40 ms with a peak normalized fluorescence of 1.36 ± 0.09 (mean ± s.d., n = 28). Spontaneous Ca2+ waves with a wide range of propagation velocities (between 30 and 260 μm s−1) were also observed. In about 60% of records (n = 33), Ca2+ sparks could be detected at the sites of wave initiation. Waves of elevated [Ca2+]i propagated with non-constant velocity and in some cases terminated. These observations could be explained by heterogeneity in the distribution of subcellular release sites as well as variability in the contribution of each release site to the wave. Spontaneous [Ca2+]i transients in single dispersed visceral smooth muscle cells have a wide spectrum of behaviour that is likely to be the result of spatio-temporal recruitment of smaller local events, probably via a calcium-induced calcium release (CICR) mechanism. The spatial non-uniformity of SR and RyR distribution within the cell may account for the existence of ‘frequent discharge sites’ firing the majority of the smooth muscle Ca2+ sparks and the wide variation in the Ca2+ wave propagation velocities observed. |
| Related Links | http://dx.doi.org/10.1111/j.1469-7793.1998.707bs.x |
| Ending Page | 720 |
| Page Count | 14 |
| Starting Page | 707 |
| File Format | |
| ISSN | 00223751 |
| e-ISSN | 14697793 |
| Journal | The Journal of Physiology |
| Issue Number | Pt 3 |
| Volume Number | 507 |
| Language | English |
| Publisher | Blackwell Science Inc |
| Publisher Date | 1998-03-01 |
| Access Restriction | Open |
| Rights Holder | Blackwell Science Inc |
| Subject Keyword | Physiology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Sports Science |
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