NDLI: Calcium cycling and contractile activation in intact mouse cardiac muscle

Content Provider	PubMed Central
Author	Gao, Wei Dong Perez, Nestor Gustavo Marban, Eduardo
Copyright Year	1998
Abstract	Excitation-contraction coupling in mouse cardiac muscle remains poorly characterized, despite the fact that the mouse is the mammalian species of choice for genetic manipulation. In this study, we characterized the relationship between internal calcium concentration ([Ca2+]i) and contraction in intact mouse ventricular muscle loaded with fura-2 salt at 20–22°C. Both Ca2+ transient amplitude and twitch force increased monotonically as external Ca2+ concentration ([Ca2+]o) was increased up to 8.0 mm, with no changes in diastolic levels or in the times to peak of either Ca2+ transients or force. The decay of Ca2+ transients was accelerated as [Ca2+]o increased, while relaxation was prolonged. Both Ca2+ transient amplitude and twitch force increased as stimulation rate increased from 0.2 to 4 Hz, but the increase in force was much greater than the underlying increase in [Ca2+]i. The steady-state force-[Ca2+]i relationship revealed an [Ca2+]i required for 50% of maximal activation (Ca50) of 0.95 ± 0.08 μm, a Hill coefficient of 9.9 ± 2.6, and a maximal Ca2+-activated force (F max) of 60 ± 5 mN mm−2. Unlike rat ventricular myocardium, mouse cardiac muscle resists supraphysiological [Ca2+]o. The strong positive force-frequency relationship in mouse cardiac muscle, with increases of force disproportionate to the increases in Ca2+ transients, suggests frequency-dependent ‘sensitization’ of the myofilaments. During steady-state activation, mouse muscle exhibits decreased Ca2+ responsiveness relative to other species, but high co-operativity. These physiological features of mouse cardiac muscle merit consideration when interpreting the phenotypic consequences of genetic manipulations in this species.
Related Links	http://dx.doi.org/10.1111/j.1469-7793.1998.175bu.x
Ending Page	184
Page Count	10
Starting Page	175
File Format	PDF
ISSN	00223751
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 1
Volume Number	507
Language	English
Publisher	Blackwell Science Inc
Publisher Date	1998-02-01
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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