NDLI: A Novel Interaction of the Golgi Complex with the Vimentin Intermediate Filament Cytoskeleton

Content Provider	PubMed Central
Author	Gao, Ya-sheng Elizabeth, Sztul
Copyright Year	2001
Abstract	The integration of the vimentin intermediate filament (IF) cytoskeleton and cellular organelles in vivo is an incompletely understood process, and the identities of proteins participating in such events are largely unknown. Here, we show that the Golgi complex interacts with the vimentin IF cytoskeleton, and that the Golgi protein formiminotransferase cyclodeaminase (FTCD) participates in this interaction. We show that the peripherally associated Golgi protein FTCD binds directly to vimentin subunits and to polymerized vimentin filaments in vivo and in vitro. Expression of FTCD in cultured cells results in the formation of extensive FTCD-containing fibers originating from the Golgi region, and is paralleled by a dramatic rearrangements of the vimentin IF cytoskeleton in a coordinate process in which vimentin filaments and FTCD integrate into chimeric fibers. Formation of the FTCD fibers is obligatorily coupled to vimentin assembly and does not occur in vim−/− cells. The FTCD-mediated regulation of vimentin IF is not a secondary effect of changes in the microtubule or the actin cytoskeletons, since those cytoskeletal systems appear unaffected by FTCD expression. The assembly of the FTCD/vimentin fibers causes a coordinate change in the structure of the Golgi complex and results in Golgi fragmentation into individual elements that are tethered to the FTCD/vimentin fibers. The observed interaction of Golgi elements with vimentin filaments and the ability of FTCD to specifically interacts with both Golgi membrane and vimentin filaments and promote their association suggest that FTCD might be a candidate protein integrating the Golgi compartment with the IF cytoskeleton.
Starting Page	877
File Format	PDF
ISSN	15408140
e-ISSN	15408140
Journal	The Journal of Cell Biology
Issue Number	5
Volume Number	152
Language	English
Publisher	The Rockefeller University Press
Publisher Date	2001-03-05
Access Restriction	Open
Rights Holder	The Rockefeller University Press
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A Novel Interaction of the Golgi Complex with the Vimentin Intermediate Filament Cytoskeleton

Functional analysis of desmoplakin domains: specification of the interaction with keratin versus vimentin intermediate filament networks

Characterization of pinin, a novel protein associated with the desmosome-intermediate filament complex

Nanomechanics of vimentin intermediate filament networks

Interaction of frog virus-3 with the cytoskeleton. I. Altered organization of microtubules, intermediate filaments, and microfilaments

Architecture of the vimentin cytoskeleton is modified by perturbation of the GTPase ARF1

The interaction in vitro of the intermediate filament protein vimentin with naturally occurring RNAs and DNAs.

Thematic Minireview Series: The State of the Cytoskeleton in 2015

Intermediate filaments.

A Novel Interaction of the Golgi Complex with the Vimentin Intermediate Filament Cytoskeleton

Similar Documents

A Novel Interaction of the Golgi Complex with the Vimentin Intermediate Filament Cytoskeleton

Functional analysis of desmoplakin domains: specification of the interaction with keratin versus vimentin intermediate filament networks

Characterization of pinin, a novel protein associated with the desmosome-intermediate filament complex

Nanomechanics of vimentin intermediate filament networks

Interaction of frog virus-3 with the cytoskeleton. I. Altered organization of microtubules, intermediate filaments, and microfilaments

Architecture of the vimentin cytoskeleton is modified by perturbation of the GTPase ARF1

The interaction in vitro of the intermediate filament protein vimentin with naturally occurring RNAs and DNAs.

Thematic Minireview Series: The State of the Cytoskeleton in 2015

Intermediate filaments.

A Novel Interaction of the Golgi Complex with the Vimentin Intermediate Filament Cytoskeleton