NDLI: The budding yeast silencing protein Sir1 is a functional component of centromeric chromatin

Content Provider	PubMed Central
Author	Sharp, Judith A. Krawitz, Denise C. Gardner, Kelly A. Fox, Catherine A. Kaufman, Paul D.
Copyright Year	2003
Abstract	In fission yeast and multicellular organisms, centromere-proximal regions of chromosomes are heterochromatic, containing proteins that silence gene expression. In contrast, the relationship between heterochromatin proteins and kinetochore function in the budding yeast Saccharomyces cerevisiae remains largely unexplored. Here we report that the yeast heterochromatin protein Sir1 is a component of centromeric chromatin and contributes to mitotic chromosome stability. Sir1 recruitment to centromeres occurred through a novel mechanism independent of its interaction with the origin recognition complex (ORC). Sir1 function at centromeres was distinct from its role in forming heterochromatin, because the Sir2-4 proteins were not associated with centromeric regions. Sir1 bound to Cac1, a subunit of chromatin assembly factor I (CAF-I), and helped to retain Cac1 at centromeric loci. These studies reveal that although budding yeast and mammalian cells use fundamentally different mechanisms of forming heterochromatin, they both use silencing proteins to attract the histone deposition factor CAF-I to centromeric chromatin.
Related Links	http://dx.doi.org/10.1101/gad.1131103
Ending Page	2361
Page Count	6
Starting Page	2356
File Format	PDF
ISSN	08909369
Journal	Genes & Development
Issue Number	19
Volume Number	17
Language	English
Publisher	Cold Spring Harbor Laboratory Press
Publisher Date	2003-10-01
Access Restriction	Open
Rights Holder	Cold Spring Harbor Laboratory Press
Subject Keyword	Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics Developmental Biology

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