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| Content Provider | PubMed Central |
|---|---|
| Author | Liang, Weili Silva, Anisia J. Benitez, Jorge A. |
| Abstract | Inactivation of the quorum-sensing regulator HapR causes Vibrio cholerae El Tor biotype strain C7258 to adopt a rugose colonial morphology that correlates with enhanced biofilm formation. V. cholerae mutants lacking the cyclic AMP (cAMP) receptor protein (CRP) produce very little HapR, which results in elevated expression of Vibrio exopolysaccharide (vps) genes and biofilm compared to the wild type. However, Δcrp mutants still exhibited smooth colonial morphology and expressed reduced levels of vps genes compared to isogenic hapR mutants. In this study we demonstrate that deletion of crp and cya (adenylate cyclase) converts a rugose ΔhapR mutant to a smooth one. The smooth ΔhapR Δcrp and ΔhapR Δcya double mutants could be converted back to rugose by complementation with crp and cya, respectively. CRP was found to enhance the expression of VpsR, a strong activator of vps expression, but to diminish transcription of VpsT. Ectopic expression of VpsR in smooth ΔhapR Δcrp and ΔhapR Δcya double mutants restored rugose colonial morphology. Lowering intracellular cAMP levels in a ΔhapR mutant by the addition of glucose diminished VpsR expression and colonial rugosity. On the basis of our results, we propose a model for the regulatory input of CRP on exopolysaccharide biosynthesis. |
| Related Links | http://dx.doi.org/10.1128/AEM.01564-07 |
| Starting Page | 7482 |
| File Format | |
| ISSN | 10985336 |
| e-ISSN | 10985336 |
| Journal | Applied and Environmental Microbiology |
| Issue Number | 22 |
| Volume Number | 73 |
| Language | English |
| Publisher | American Society for Microbiology (ASM) |
| Publisher Date | 2007-11-01 |
| Access Restriction | Open |
| Rights Holder | American Society for Microbiology (ASM) |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ecology Food Science Applied Microbiology and Biotechnology Biotechnology |
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