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| Content Provider | PubMed Central |
|---|---|
| Author | Yoch, Duane C. |
| Abstract | Nitrogenase (N2ase) from the photosynthetic bacterium Rhodospirillum rubrum can exist in two forms, an unregulated form (N2ase A) and a regulatory form (N2ase R), the latter being identified in vitro by its need for activation by a Mn2+-dependent N2ase activating system. The physiological significance of this Mn2+-dependent N2ase activating system was suggested here by observations that growth of R. rubrum and Rhodopseudomonas capsulata on N2 gas (a condition that produces active N2ase R) required Mn2+, but growth on ammonia or glutamate did not. Manganese could not be shown to be required for the biosynthesis of either nitrogenase or glutamine synthetase or for glutamine synthetase turnover, but it was required for the in vitro activation of N2ases from N2 and glutamate-grown R. rubrum and R. capsulata cells. Chromatium N2ase, in contrast, was always fully active and did not require Mn2+ activation, suggesting that only the purple nonsulfur bacteria are capable of controlling their N2ase activity by this new type of regulatory system. Although R. rubrum could not substitute Fe2+ for Mn2+ in the in vivo N2 fixation process, Fe2+ and, to a lesser extent, Co2+ could substitute for Mn2+ in the in vitro activation of N2ase. Electron paramagnetic resonance spectroscopy of buffer-washed R. rubrum chromatophores showed lines characteristic of Mn2+. Removal of the Mn2+-dependent N2ase activating factor by a salt wash of the chromatophores removed 90% of the Mn2+, which suggested a specific coupling of this metal to the activating factor. The data presented here all indicate that Mn2+ plays an important physiological role in regulating the N2 fixation process by these photosynthetic bacteria. |
| Starting Page | 987 |
| File Format | |
| ISSN | 10985530 |
| e-ISSN | 10985530 |
| Journal | Journal of Bacteriology |
| Issue Number | 3 |
| Volume Number | 140 |
| Language | English |
| Publisher Date | 1979-12-01 |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Molecular Biology Microbiology |
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