NDLI: A study of 7-deaza-2′-deoxyguanosine–2′-deoxycytidine base pairing in DNA

Content Provider	PubMed Central
Author	Ganguly, Manjori Wang, Feng Kaushik, Mahima Stone, Michael P. Marky, Luis A. Gold, Barry
Copyright Year	2007
Abstract	The incorporation of 7-deazaguanine modifications into DNA is frequently used to probe protein recognition of H-bonding information in the major groove of DNA. While it is generally assumed that 7-deazaguanine forms a normal Watson–Crick base pair with cytosine, detailed thermodynamic and structural analyses of this modification have not been reported. The replacement of the 7-N atom on guanine with a C–H, alters the electronic properties of the heterocycle and eliminates a major groove cation-binding site that could affect the organization of salts and water in the major groove. We report herein the characterization of synthetic DNA oligomers containing 7-deazaguanine using a variety of complementary approaches: UV thermal melting, differential scanning calorimetry (DSC), circular dichroism (CD), chemical probing and NMR. The results indicate that the incorporation of a 7-deazaguanine modification has a significant effect on the dynamic structure of the DNA at the flanking residue. This appears to be mediated by changes in hydration and cation organization.
Related Links	http://dx.doi.org/10.1093/nar/gkm670
Ending Page	6195
Page Count	15
Starting Page	6181
File Format	PDF
ISSN	03051048
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	18
Volume Number	35
Language	English
Publisher	Oxford University Press
Publisher Date	2007-08-01
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Genetics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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