NDLI: A force-based protein biochip

Content Provider	PubMed Central
Author	Blank, K. Mai, T. Gilbert, I. Schiffmann, S. Rankl, J. Zivin, R. Tackney, C. Nicolaus, T. Spinnler, K. Oesterhelt, F. Benoit, M. Clausen-schaumann, H. Gaub, H. E.
Copyright Year	2003
Abstract	A parallel assay for the quantification of single-molecule binding forces was developed based on differential unbinding force measurements where ligand–receptor interactions are compared with the unzipping forces of DNA hybrids. Using the DNA zippers as molecular force sensors, the efficient discrimination between specific and nonspecific interactions was demonstrated for small molecules binding to specific receptors, as well as for protein–protein interactions on protein arrays. Finally, an antibody sandwich assay with different capture antibodies on one chip surface and with the detection antibodies linked to a congruent surface via the DNA zippers was used to capture and quantify a recombinant hepatitis C antigen from solution. In this case, the DNA zippers enable not only discrimination between specific and nonspecific binding, but also allow for the local application of detection antibodies, thereby eliminating false-positive results caused by cross-reactive antibodies and nonspecific binding.
Related Links	http://dx.doi.org/10.1073/pnas.1934928100
Ending Page	11360
Page Count	5
Starting Page	11356
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	20
Volume Number	100
Language	English
Publisher	National Academy of Sciences
Publisher Date	2003-09-30
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A force-based protein biochip.

Entropy-driven collective interactions in DNA brushes on a biochip

Physics-based protein-structure prediction using a hierarchical protocol based on the UNRES force field: Assessment in two blind tests

Protein model refinement using an optimized physics-based all-atom force field

Evaluating and optimizing computational protein design force fields using fixed composition-based negative design

Atomic force microscopy reveals the mechanical design of a modular protein

Monocyte-induced recovery of inflammation-associated hepatocellular dysfunction in a biochip-based human liver model

Probing ribosomal protein–RNA interactions with an external force

Direct observation of a force-induced switch in the anisotropic mechanical unfolding pathway of a protein

A force-based protein biochip

Similar Documents

A force-based protein biochip.

Entropy-driven collective interactions in DNA brushes on a biochip

Physics-based protein-structure prediction using a hierarchical protocol based on the UNRES force field: Assessment in two blind tests

Protein model refinement using an optimized physics-based all-atom force field

Evaluating and optimizing computational protein design force fields using fixed composition-based negative design

Atomic force microscopy reveals the mechanical design of a modular protein

Monocyte-induced recovery of inflammation-associated hepatocellular dysfunction in a biochip-based human liver model

Probing ribosomal protein–RNA interactions with an external force

Direct observation of a force-induced switch in the anisotropic mechanical unfolding pathway of a protein

A force-based protein biochip