NDLI: Degradation of Carbazole by Microbial Cells Immobilized in Magnetic Gellan Gum Gel Beads▿

Content Provider	PubMed Central
Author	Wang, Xia Gai, Zhonghui Yu, Bo Feng, Jinhui Xu, Changyong Yuan, Yong Lin, Zhixin Xu, Ping
Copyright Year	2007
Abstract	Polycyclic aromatic heterocycles, such as carbazole, are environmental contaminants suspected of posing human health risks. In this study, we investigated the degradation of carbazole by immobilized Sphingomonas sp. strain XLDN2-5 cells. Four kinds of polymers were evaluated as immobilization supports for Sphingomonas sp. strain XLDN2-5. After comparison with agar, alginate, and κ-carrageenan, gellan gum was selected as the optimal immobilization support. Furthermore, Fe3O4 nanoparticles were prepared by a coprecipitation method, and the average particle size was about 20 nm with 49.65-electromagnetic-unit (emu) g−1 saturation magnetization. When the mixture of gellan gel and the Fe3O4 nanoparticles served as an immobilization support, the magnetically immobilized cells were prepared by an ionotropic method. The biodegradation experiments were carried out by employing free cells, nonmagnetically immobilized cells, and magnetically immobilized cells in aqueous phase. The results showed that the magnetically immobilized cells presented higher carbazole biodegradation activity than nonmagnetically immobilized cells and free cells. The highest biodegradation activity was obtained when the concentration of Fe3O4 nanoparticles was 9 mg ml−1 and the saturation magnetization of magnetically immobilized cells was 11.08 emu g−1. Additionally, the recycling experiments demonstrated that the degradation activity of magnetically immobilized cells increased gradually during the eight recycles. These results support developing efficient biocatalysts using magnetically immobilized cells and provide a promising technique for improving biocatalysts used in the biodegradation of not only carbazole, but also other hazardous organic compounds.
Related Links	http://dx.doi.org/10.1128/AEM.01051-07
Starting Page	6421
File Format	PDF
ISSN	10985336
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	20
Volume Number	73
Language	English
Publisher	American Society for Microbiology (ASM)
Publisher Date	2007-10-01
Access Restriction	Open
Rights Holder	American Society for Microbiology (ASM)
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

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