NDLI: Advanced Environmental Surveillance and Molecular Analyses Indicate Separate Importations Rather than Endemic Circulation of Wild Type 1 Poliovirus in Gaza District in 2002▿

Content Provider	PubMed Central
Author	Manor, Y. Blomqvist, S. Sofer, D. Alfandari, J. Halmut, T. Abramovitz, B. Mendelson, E. Shulman, L. M.
Copyright Year	2007
Abstract	An improved sewage surveillance algorithm (sample acquisition, processing, and molecular analysis) for wild and vaccine-derived polioviruses was developed and validated. It was based on plaque isolation with sensitive and high-throughput methods. The molecular analysis included sequencing; a comparison of the type, rate, and distribution of nucleotide substitutions with a profile for outbreaks evolving from a single progenitor; and phylogenetic analysis for relative similarity. The analyses revealed that two environmental wild type 1 isolates from the Gaza district in 2002 were imported separately, most likely from Egyptian southern governorates, and were not linked by endemic circulation. These findings illustrate the continuous spreading potential of wild-type poliovirus and underscore the value of extensive environmental surveillance employing advanced molecular analysis to monitor wild poliovirus in poliomyelitis-free regions.
Related Links	http://dx.doi.org/10.1128/aem.02537-06
Ending Page	5958
Page Count	5
Starting Page	5954
File Format	PDF
ISSN	00992240
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	18
Volume Number	73
Language	English
Publisher	American Society for Microbiology (ASM)
Publisher Date	2007-09-15
Access Restriction	Open
Rights Holder	American Society for Microbiology (ASM)
Subject Keyword	Biotechnology Food Science Ecology Applied Microbiology and Biotechnology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

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