NDLI: Hypomorphic mutation in an essential cell-cycle kinase causes growth retardation and impaired spermatogenesis

Content Provider	PubMed Central
Author	Kim, Jung Min Takemoto, Naofumi Arai, Ken-ichi Masai, Hisao
Copyright Year	2003
Abstract	Cdc7 kinase is essential for initiation of DNA replication. Cdc7 –/– mouse embryonic stem (ES) cells are non-viable but their growth can be rescued by an ectopically expressed transgene (Cdc7 –/–tg). Here we report that, despite the normal growth capability of Cdc7 –/–tg ES cells, the mice with the identical genetic background exhibit growth retardation. Concomi tantly, Cdc7 –/–tg embryonic fibroblasts (MEFs) display delayed S phase entry and slow S phase progression. Furthermore, spermatogenesis of Cdc7 –/–tg mice is disrupted prior to pachytene stage of meiotic prophase I. The impairment in spermatogenesis correlates with the extremely low level of Cdc7 protein in testes, and is rescued by introducing an additional allele of transgene, which results in increase of Cdc7 expression. The increased level of Cdc7 also recovers the growth of Cdc7 –/–tg MEFs and mice, indicating that the developmental abnormalities of Cdc7 –/–tg mice are due to insufficiency of Cdc7 protein. Our results indicate the requirement of a critical level of a cell-cycle regulator for mouse development and provide genetic evidence that Cdc7 plays essential roles in meiotic processes in mammals.
Related Links	http://dx.doi.org/10.1093/emboj/cdg497
Ending Page	5272
Page Count	13
Starting Page	5260
File Format	PDF
ISSN	14602075
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	19
Volume Number	22
Language	English
Publisher	Oxford University Press
Publisher Date	2003-10-01
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

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