NDLI: Ionic effects on viral DNA packaging and portal motor function in bacteriophage φ29

Content Provider	PubMed Central
Author	Fuller, Derek N. Peter, Rickgauer John Jardine, Paul J. Grimes, Shelley Anderson, Dwight L. Smith, Douglas E.
Copyright Year	2007
Abstract	In many viruses, DNA is confined at such high density that its bending rigidity and electrostatic self-repulsion present a strong energy barrier in viral assembly. Therefore, a powerful molecular motor is needed to package the DNA into the viral capsid. Here, we investigate the role of electrostatic repulsion on single DNA packaging dynamics in bacteriophage φ29 via optical tweezers measurements. We show that ionic screening strongly affects the packing forces, confirming the importance of electrostatic repulsion. Separately, we find that ions affect the motor function. We separate these effects through constant force measurements and velocity versus load measurements at both low and high capsid filling. Regarding motor function, we find that eliminating free Mg2+ blocks initiation of packaging. In contrast, Na+ is not required, but it increases the motor velocity by up to 50% at low load. Regarding internal resistance, we find that the internal force was lowest when Mg2+ was the dominant ion or with the addition of 1 mM Co3+. Forces resisting DNA confinement were up to ≈80% higher with Na+ as the dominant counterion, and only ≈90% of the genome length could be packaged in this condition. The observed trend of the packing forces is in accord with that predicted by DNA charge-screening theory. However, the forces are up to six times higher than predicted by models that assume coaxial spooling of the DNA and interaction potentials derived from DNA condensation experiments. The forces are also severalfold higher than ejection forces measured with bacteriophage λ.
Related Links	http://dx.doi.org/10.1073/pnas.0701323104
Ending Page	11250
Page Count	6
Starting Page	11245
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	27
Volume Number	104
Language	English
Publisher	National Academy of Sciences
Publisher Date	2007-07-03
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Portal Motor Velocity and Internal Force Resisting Viral DNA Packaging in Bacteriophage φ29

Ionic effects on viral DNA packaging and portal motor function in bacteriophage φ29

Affinity of molecular interactions in the bacteriophage φ29 DNA packaging motor

Affinity of molecular interactions in the bacteriophage φ29 DNA packaging motor

Function of a viral genome packaging motor from bacteriophage T4 is insensitive to DNA sequence

Direct Interaction of the Bacteriophage SPP1 Packaging ATPase with the Portal Protein

Substrate Interactions and Promiscuity in a Viral DNA Packaging Motor

Structure of the bacteriophage ϕ29 DNA packaging motor

Strong subunit coordination drives a powerful viral DNA packaging motor.

Ionic effects on viral DNA packaging and portal motor function in bacteriophage φ29

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Portal Motor Velocity and Internal Force Resisting Viral DNA Packaging in Bacteriophage φ29

Ionic effects on viral DNA packaging and portal motor function in bacteriophage φ29

Affinity of molecular interactions in the bacteriophage φ29 DNA packaging motor

Affinity of molecular interactions in the bacteriophage φ29 DNA packaging motor

Function of a viral genome packaging motor from bacteriophage T4 is insensitive to DNA sequence

Direct Interaction of the Bacteriophage SPP1 Packaging ATPase with the Portal Protein

Substrate Interactions and Promiscuity in a Viral DNA Packaging Motor

Structure of the bacteriophage ϕ29 DNA packaging motor

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Ionic effects on viral DNA packaging and portal motor function in bacteriophage φ29